RNACell-Free RNA6Microbial RNA7miRNA10mRNA9RNA Clean Up3Total RNA31miRNeasy Tissue/Cells Advanced Mini KitPrint For phenol-free purification of microRNA and total RNA from tissues and cellsPhenol-free protocol – no need for phase separation or working under a hoodFast and convenient procedure due to the advanced chemistry and gDNA Eliminator ColumnsEfficient enrichment of miRNA and RNAs <200 nucleotidesConsistent, pure RNA for all downstream applicationsThe miRNeasy Tissue/Cells Advanced Mini Kit is the second generation of the well-established miRNeasy Mini Kit. This improved version combines a phenol-free advanced chemistry with gDNA Eliminator Columns for safe and convenient RNA isolation. miRNeasy Tissue/Cells Advanced Mini Kit enables purification of total RNA, including small RNAs ≥18 nucleotides, from animal tissues and cells. The kit uses spin columns for low-throughput RNA purification. Furthermore, the entire procedure can be automated on QIAcube Connect.Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:217604miRNeasy Tissue/Cells Advanced Mini Kit (50)$369.00Order ProductFor 50 preps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and BuffersProduct Details Total RNA extraction: effective genomic DNA removal Total RNA was purified from 10 mg RNAlater-stabilized rat tissue (liver, muscle, brain, thymus and kidney) using the miRNeasy Tissue/Cells Advanced Mini Kit andthe miRNeasy Mini Kit. Real-time RT-PCR assays for pgk1 were performed with (+RT, dark bars) or without (–RT, light bars) reverse transcriptase. CT values of samples treated with reverse transcriptase were comparable for both kits and samples not treated with reverse transcriptase showed better performance with the miRNeasy Tissue/Cells Advanced Mini Kit compared to the miRNeasy Mini Kit.Fast procedureThe time needed to isolate RNA from 21 samples were compared among the miRNeasy Tissue/Cells Advanced Mini Kit and 6 different kits from other suppliers. The miRNeasy Advanced Tissue/Cells Mini Kit was one of the fasted kits.Competitive performanceTotal RNA including small RNA was isolated from 5 mg stabilized rat tissue (liver, brain, heart, kidney and lung) using the miRNeasy Tissue/Cells Advanced Mini Kit and 6 different kits from other suppliers. Four microliters of recovered RNA, in a total volume of 20 µl using the miRCURY System, was used for miRNA detection on the miR-16. The miRNeasy Tissue/Cells Advanced Mini Kit shows a comparable or better performance than kits from other suppliers.High recovery of miRNA without the need for phenolTotal RNA including small RNA was isolated from 20 mg frozen rat tissue (kidney, liver, thymus, brain and muscle) using the miRNeasy Tissue/Cells Advanced Mini Kit (blue bars) and the miRNeasy Mini Kit (red bars). Five microliters of recovered RNA, in a total volume of 25 µl, was used for miRNA detection on the miR16. Both kits show comparable performance, even for difficult tissue types like muscle or brain.PerformanceThe miRNeasy Tissue/Cells Advanced Mini Kit is a phenol-free alternative to the miRNeasy Mini Kit with similar RNA yields and quality (see figure "High recovery of miRNA without the need for phenol"), and enables efficient enrichment of RNA down to approximately 18 nucleotides in size. The miRNeasy kits efficiently purify RNA from up to 30 mg frozen tissue or 1 x 107 cells, but also works with lower amounts of starting material. The miRNeasy Tissue/Cells Advanced Mini Kit includes the gDNA Eliminator procedure allowing high, reproducible RNA yields and efficient genomic DNA elimination for sensitive applications (see figure "Total RNA extraction: effective genomic DNA removal"). Total RNA with Agilent RIN values close to 10 is routinely obtained from tissues and cultured cells. Unlike kits from other suppliers, the miRNeasy Tissue/Cells Advanced Mini Kit combines ease-of-use and a phenol-free protocol without any compromise on RNA quality or yield (see figures "Competitive performance" and "Fast procedure").PrincipleThe miRNeasy Tissue/Cells Advanced Mini Kit combines a guanidine-based sample lysis with an inhibitor removal step and a silica-membrane-based purification of total RNA, which includes miRNA and other small RNA. Buffer RLT, included in the kit, contains guanidine thiocyanate which facilitates lysis and denaturation of RNases and other proteins. Therefore, RNA in samples lysed in buffer RLT are stable and protected from degradation.ProcedureSample lysis is achieved by adding Buffer RLT to tissue or cell samples followed by a thorough disruption and homogenization step to ensure complete lysis and inactivation of RNases. Next, Buffer AL is added to the lysate to optimize gDNA removal by the gDNA Eliminator Spin Column. Depending on the type and amount of starting material, an optional on column DNase digestion can be carried out (not included in the kit). Buffer RPP is added to the flow-through to precipitate inhibitors (mostly proteins that are highly concentrated in tissue and cell samples and might interfere with the RNA isolation or the downstream analysis) by centrifugation. The supernatant containing the total RNA is transferred into a new reaction tube, and isopropanol is added to provide appropriate binding conditions for all RNA molecules ≥18 nucleotides. The sample is then applied to a RNeasy Mini Spin Column, where the total RNA binds to the membrane and all contaminants are efficiently washed away. High-quality RNA, including miRNA and other small RNA, are eluted in a small volume of RNase-Free Water..ApplicationsThe miRNeasy Tissue/Cells Advanced Mini Kit allows purification of miRNA and total RNA for a variety of applications, including:Quantitative, real-time RT-PCRRNA-seqMicroarray analysisNorthern blot analysisSpecificationsFeaturesSpecifications ApplicationsNext-generation sequencing, real-time RT-PCR, microarray Elution volume30 µl FormatSpin column Main sample typeTissue, cells ProcessingManual (centrifugation) Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinmiRNA, total RNA Sample amountup to 30 mg frozen tissue (15 mg stabilized), up to 1 x 10e7 cells TechnologySilica technology YieldVariesProduct ResourcesYou are not authorized to download the resourceFAQs (11) Sort optionsSort alphabetically What can I use to isolate RNA smaller than 200 nucleotides?FAQ ID -115ViewHow can I check the integrity of RNA purified using RNeasy Kits?FAQ ID -1024ViewDo I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?FAQ ID -1037ViewEffects of low A260/A230 ratios in RNA preparations on downstream applicationsFAQ ID -2248ViewCan I use total RNA for the miRNA PCR Arrays or Assays?FAQ ID -2726ViewWhat is the composition of Buffer RPE? FAQ ID -2797ViewWhat is the composition of Buffer RWT? FAQ ID -2798ViewHow much RNA does a typical mammalian cell contain? FAQ ID -2946ViewWhy is the protein precipitation step optional when working with cells?FAQ ID-3752ViewHow do I clean up RNA preparations containing miRNA?FAQ ID -3002ViewWhy do I have to add Buffer AL before using the gDNA Eliminator?FAQ ID-3751ViewShow allShow lessQuick-Start Protocols (1) Sort optionsSort alphabetically miRNeasy Tissue/Cells Advanced Mini Kit Quick-Start ProtocolShow detailsShow allShow lessBrochures & Guides (1) Sort optionsSort alphabetically iPP miRNeasy Tissue/Cells Advanced MiniShow detailsShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically miRNeasy Tissue/Cells Advanced Mini Kit HandbookShow detailsShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:217204miRNeasy Serum/Plasma Advanced Kit (50)For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-Free Reagents and Buffers$461.00Add To CartCat No./ID:339371miRCURY LNA Probe PCR Kit (200)For 200 reactions: 2X QuantiNova Probe Master Mix, 10X miRCURY Probe Univ. 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