BackgroundBuffer & StorageReviews Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 stability during mitosis.Human recombinant EB3 is a full length protein with an N-terminal His-tag (34 kDa). The protein is detected by rabbit polyclonal anti-EB3 (a.a. 171-182) (Cat.#EP5121) and rat monoclonal EB3 (Cat.#EM5101). ReferencesKomarova, Y. et al. (2012) Mol Cell. 48:914. Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1.Komarova, Y. et al. (2005) Mol Biol Cell. 16(11):5334. EB3 recombinant protein lysate is supplied at a concentration of 5 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year. The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.This kit contains:KIT SUMMARY