BackgroundSequence SpecificityBuffer & StorageReviews α-Actinins are widely expressed cytoskeletal proteins that cross-link actin filaments through anti-parallel homodimers of the rod domains. Four α-actinin genes have been discovered in humans with α-actinin 1 and 4 being widely expressed in non-muscle cells. α-Actinins contain three conserved domains that include an N-terminal actin binding domain, four spectrin-like repeats in the central region, and a C-terminal calmodulin binding domain. α-Actinin cross-links the actin filament networks and associates the network to focal adhesion sites through binding of talin and vinculin. α-Actinin 1 is phosphorylated at Tyr-12 by FAK, while α-actinin 4 can be phosphorylated at Tyr-4 and Tyr-31 after EGF treatment. Tyr-4 and Tyr-31 phosphorylation inhibit actin binding and reduces actin-filament driven multi-nucleation in rat kidney cells. Thus, phosphorylation in α-actinins may be important for regulating actin binding and actin cytoskeletal remodeling.  ReferencesShao, H. et al. (2010) J Biol Chem. 285(4):2591. Izaguirre, G. et al. (2001) J Biol Chem. 276(31):28676.Ylanne et al. (2001) Structure. 9:597. α-Actinin 4 (a.a. 2-11) synthetic peptide corresponds to amino acids in the N-terminus of human α-actinin 4. This sequence is well conserved in rat and mouse α-actinin 4, but is not conserved in other α-actinins.*For more information, see UniProt Accession O43707Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year. The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.The peptide is specifically recognized by anti-α-actinin 4 (a.a. 2-11) antibody (AP4271) in ELISA, and has been shown to block the reactivity of AP4271 during Western blot. In addition, the peptide is recommended for use in blocking AP4271 reactivity in immunocytochemistry.*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.This kit contains:KIT SUMMARY