Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (TCM)Home / Products / Assay Kits / ELISAs / Human Interferon ELISAs / Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (TCM)Product Features:Looking for the full protocol? We"re going GREEN! Click on the "Documentation" tab below! Most sensitive ELISA on the market for global IFN alpha detection in tissue culture media (TCM)Low pg/ml detection of ALL human IFN-α subtypesReproducible results with < 10% inter- and < 8% intra-assay CVsFull Product Name: VeriKine-HS Human Interferon Alpha All Subtype TCM ELISA Kit Looking for an autoimmune disease sera & TCM compatible All Subtype ELISA? Check out our 41115 kit! OrderingCatalog No. Pack Size Price Quantity 41135-1 1 x 96-well plate$395.00 SpecificationsTech Info / DataCitationsDocumentationSpecificationsCompatibility: Tissue Culture MediaELISA Assay Range: 1.95 - 125 pg/mlAssay Length: 20 hours 30 minutes - 24 hours 30 minutesSpecificity: Human Interferon AlphaCross-reacts with Cynomolgus/Rhesus IFN-α (13%). No cross-reactivity against human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; pig IFN-α; or bovine IFN-τ. Inter-Assay CV: ≤ 10%Intra-Assay CV: ≤ 8%Average Spike Recovery: 105% Storage: 2-8°CExpiration Date: One year from date of manufactureShipping Condition: Wet IceMaterials Provided:Pre-coated microtiter platePlate SealersWash Solution ConcentrateHuman Interferon Alpha Standard, 10,000 pg/mlAssay BufferStandard DiluentAntibody DiluentAntibody ConcentrateHRP Conjugate ConcentrateHRP DiluentTMB Substrate SolutionStop Solution Tech Info / Data Tips, Tools and Troubleshooting:Human IFN-α ELISA Subtype Specificities Comparison ChartDetailed Related Product Information:Quantitative Immunoassays: Single Analyte Screening Services Background:PBL’s VeriKine-HS Human Interferon Alpha All Subtype TCM ELISA Kit is specifically formulated to detect all human IFN-Alpha subtypes. This kit quantitates all human IFN alpha subtypes in tissue culture media (TCM) using a sandwich immunoassay format. The kit is based on an ELISA with biotinylated-detection antibody and streptavidin-conjugated horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha provided by the National Institutes of Health.Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. They are synthesized and secreted by most cell types in response to pathogens. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory, and many other activities. In humans, IFN-α refers to a family of proteins comprised of 12 highly homologous protein subtypes (greater than 85% by amino acid sequence), encoded by 13 genes, that exhibit pleiotropic biologic activities. Measuring the levels of the 12 different human IFN-α subtypes is essential for understanding their triggers and subsequent effects on the immune system. The ability to measure all the subtypes may provide an indication of the total IFN-α level. CitationsReferences:Staehelin et al. (1981) "A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies" in Methods in Enzymology, Vol. 79 (Pestka, ed.), Academic Press, New York, 589-595.Kelder et al. (1986) "A Sandwich Radioimmunoassay for Human IFNa" in Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 582-587.Human IFN-α international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka (1986) "Interferon Standards and General Abbreviations" in Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 14-23.Rubinstein et al. (1981) "Human Leukocyte Interferon: Isolation and Characterization of Several Molecular Forms," Arch. Biochem. Biophys. 210, 307-318.Hobbs et al. (1982) "Purification and Characterization of Interferons from a Continuous Myeloblastic Cell Line," J. Biol. Chem. 257, 4071-4076. Documentation 41135 Certificate of Analysis & Protocol (One-Page) 41135 Technical Data Sheet 41135 Protocol (Full) 41135 Certificate of Analysis (CoA) Safety Data Sheet (SDS) 41135 Flyer Typical Standard Curves in Various Matrices using PBL"s High Sensitivity Human IFN Alpha TCM ELISA (41135)