Human Type 1 IFN Neutralizing Antibody MixtureHome / Products / Antibodies / Monoclonal Antibodies (MAbs) / Anti-Human IFN MAbs / Human Type 1 IFN Neutralizing Antibody MixtureProduct Features:Mixture of monoclonal and polyclonal antibodies directed against human Type 1 Interferons (IFNs) and Type 1 Receptor Subunit 2 (IFNAR2)Effectively neutralizes biological activity of human IFN-alpha, IFN-beta, IFN-omega, IFN-kappa, and IFN-epsilonImproves neutralization of multiple Type I IFNs often found in complex samples by employing a broadly neutralizing custom antibody cocktailUnconjugated OrderingCatalog No. Pack Size Price Quantity 39000-1 0.2 ml$570.00 SpecificationsTech Info / DataCitationsDocumentationSpecificationsFormulationSupplied frozen in phosphate-buffered saline (PBS)PurificationA combination of Protein G affinity chromatography and other conventional chromatographical methods.Endotoxin level < 1 EU/μgBioactivityEncephalomyocarditis Virus (EMCV) was used to challenge A549 cells which were incubated with a constant concentration of the antibody mixture and the titrated interferon.SpecificityBlocks biological activity of human Type I interferons (alpha, beta, omega, kappa, epsilon)No cross reactivity observed with the following human interferons: gamma, lambda 1, lambda 2, lambda 3; or mouse interferons: alpha, beta, gammaStorageFor retention of full activity store at -70°C or below and avoid repeated freeze/thaw cyclesSynonymsType I interferon, Type I IFN, Neutralizing, Neutralization, Type I Interferon Receptor, Antibody Cocktail Tech Info / DataTested Applications:Neutralization Citations3 Citations:Lundberg, Rickard, et al. (2019). Zika Virus Non-Structural Protein NS5 Inhibits the RIG-I Pathway and Interferon Lambda 1 Promoter Activation by Targeting IKK Epsilon. Viruses, 20 pgs. PMID: 31690057. (link)Szabo, Peter, et al. (2019). Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease. Nature Communications, 16 pgs. PMID: 31624246. (link)Kuse, Nozomi, et al. (2019). Priming of HIV-1-specific CD8+ T cells with strong functional properties from naïve T cells. EBioMedicine, 11 pgs. PMID: 30956171. (link) Documentation 39000-1 Certificate of Analysis (CoA) SDS Representative Neutralization Curves Representative neutralization curves of Human Type I IFN Neutralizing Antibody Mixture (Catalog No. 39000) (diluted 1:50) to human interferon alpha, beta, and omega. In this assay, A549 cells (human lung carcinoma) were incubated with the antibody mixture and titrated interferon, followed by challenging with encephalomyocarditis virus (EMCV).