Anti-Human ISG15, Clone 2.1 (MAb)Home / Products / Antibodies / Monoclonal Antibodies (MAbs) / Anti-Human IFN MAbs / Anti-Human ISG15, Clone 2.1 (MAb)Product Features:Detects conjugated and free human ISG15No cross-reactivity with 1000x load of ubiquitin on Western BlotGreater than 95% purity OrderingCatalog No. Pack Size Price Quantity 21900-1 100 μg$255.00 SpecificationsTech Info / DataCitationsDocumentationSpecificationsFormulation50 mM Tris HCl, 75 mM NaCl, 50% Glycerol pH 8AntigenHuman ISG15IsotypeMouse IgG1 kappaHost SpeciesMousePurity> 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μgSpecificityDetects human ISG15StorageAfter receipt, this product should be kept at -20oC for retention of full activity. Do not store below -20oC.Clone2.1 Tech Info / DataTested Applications:Western Blot Background:Many published reports indicate that ISG15 (G1P2) is commonly upregulated in response to Type I interferon stimulation. Though a wide range of cellular activites are influenced by ISG15 expression including Type I interferon signaling, translation, chromatin remodeling, cell motility, protein trafficking, and protein conjugation (ISGylation), the complete spectrum of ISG15-dependent biological sequelae remains to be fully elucidated. Further definition of new ISG15 target proteins and the role of free ISG15 will offer new insights into mechanistic and immunotherapeutic approaches to human diseases. CitationsReferencesN Arnaud, S Dabo, D Akazawa, M Fukasawa, F Shinkai-Ouchi, J Hugon, T Wakita, and EF Meurs. "Hepatitis C Virus Reveals a Novel Early Control in Acute Immune Response," PLoS Pathog, 2011, 7(10): e1002289.C Dieterich and DA Relman. "Modulation of the Host Interferon Response and ISGylation Pathway by B. pertussis Filamentous Hemagglutinin," PLoS ONE, 2011, 6(11): e27535.YK Zhang, YL Wang, and JF Gui. "Identification and characterization of two homologues of interferon-stimulated gene ISG15 in crucian carp," Fish and Shellfish Immunology, 2007, 23(1): 52-61.T Takeuchi, T Kobayashi, S Tamura, and H Yokosawa. "Negative regulation of protein phosphatase 2Cβ by ISG15 conjugation," FEBS Letters, 2006, 580(18): 4521-4526.MP Malakhov, K II Kim, OA Malakhova, BS Jacobs, EC Borden, and D Zhang. "High-throughput Immunoblotting: Ubiquitin-like Protein ISG15 Modifies Key Regulators of Signal Transduction," JBC, 2003, 278, 16608-16613.J D"Cunha, S Ramanujam, RJ Wagner, PL Witt, E Knight, Jr. and EC Borden. "In vitro and in vivo secretion of human ISG15, an IFN-induced immunomodulatory cytokine," Journal of Immunology, 1996, 157:4100.J D"Cunha, E Knight, Jr., AL Hass, RL Trutt and EC Borden. "Immunoregulatory properties of ISG15, an interferon-induced cytokine," PNAS, 1996, 93:211. Documentation 21900-1 Certificate of Analysis (CoA) MSDS Product Flyer Western Blot Control (C) and Treated (T) 40 μg lysate samples from A549, Ovcar and HeLa cells were run alongside 50 ng recombinant ISG15 and 100 μg ubiquitin on a 12% SDS-PAGE gel under reducing conditions. Cells were either untreated (C) or treated (T) with 1000 U/ml of Human IFN beta 1a (PBL Cat. No. 11410-2) and lysed with 50 mM Tris-HCl pH8, 200 mM NaCl, 10% Glycerol, 0.5% NP-40, 0.1 mM EDTA with protease inhibitors on ice for one hour and then centrifuged at 4°C to obtain supernatants. No cross-reactivity was observed with ubiquitin at tested concentrations up to 100 μg. The antibody was additionally tested on Daudi and U937 cell lysates (data not shown).*A549 FT: Lysate after one freeze-thaw cycle