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PBL/Mouse Monoclonal Antibody against Human IFNAR1, Clone MMHAR-3 (MAb)/50 µg/21370-1
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Mouse Monoclonal Antibody against Human IFNAR1, Clone MMHAR-3 (MAb)Home / Products / Antibodies / Monoclonal Antibodies (MAbs) / Anti-Human IFN MAbs / Mouse Monoclonal Antibody against Human IFNAR1, Clone MMHAR-3 (MAb)Product Features:Mouse Anti-Human IFN Alpha/Beta Receptor 1 (IFNAR1) (Clone MMHAR-3)Suitable for use in Flow Cytometry and Direct Binding ELISADetects human IFNAR1 extracellular domainUnconjugatedFull Product Name: Mouse Monoclonal Antibody against Human Interferon Alpha/Beta Receptor 1, Clone MMHAR-3 (MAb) OrderingCatalog No. Pack Size Price Quantity 21370-1 50 µg$295.00 SpecificationsTech Info / DataCitationsDocumentationSpecificationsFormulationPhosphate-buffered saline (PBS)AntigenU266 Myeloma CellsIsotypeMouse IgG1, kappaHost SpeciesMousePurity> 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μgSpecificityDetects human IFNAR1StorageAfter receipt, store at -20oC for short-term use (≤6 months).For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cyclesCloneMMHAR-3 Tech Info / Data Protocols and Guides:General Procedure for Cell Surface Staining using 21370-1: Mouse MAb to Human IFN Alpha/Beta Receptor 1 (link)Tested Applications:Direct Binding ELISAFlow Cytometry CitationsReferencesColamonici OR, D"Alessandro F, Diaz MO, Gregory SA, Neckers LM, Nordan R. Characterization of three monoclonal antibodies that recognize the interferon alpha 2 receptor. 1990. Proc Natl Acad Sci USA. 87(18):7230-4.Navarro S, Colamonici OR, Llombart-Bosch A. Immunohistochemical detection of the type I interferon receptor in human fetal, adult, and neoplastic tissues. 1996. Mod Pathol. 9(2):150-6. Documentation 21370-1 Certificate of Analysis (CoA) Product Flyer SDS Representative Binding Curves (ELISA) Representative binding curve of antibody to mammalian expressed human interferon alpha/beta R1 protein and to 1% BSA/PBS in a Direct Binding ELISA. High-binding polystyrene plates were coated with either 1 μg/ml of human interferon alpha/beta R1 protein or 1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Goat anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3,3",5,5"-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.
来自PBL的iLite细胞基于常规的基因报告基因检测方法,并通过使用靶标特异性报告基因构建体进行工程改造。它们为定量测定生物活性干扰素水平提供了一种简单且经济高效的方法。
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