Anti-Human IFN Beta Antibody, Clone MMHB-2 (MAb)Home / Products / Antibodies / Monoclonal Antibodies (MAbs) / Anti-Human IFN MAbs / Anti-Human IFN Beta Antibody, Clone MMHB-2 (MAb)Product Features:Mouse monoclonal antibody against Human IFN Beta (Clone MMHB-2)Suitable for use in neutralization, direct ELISA and Western BlotUnconjugated OrderingCatalog No. Pack Size Price Quantity 21410-1 500 μg$1,715.00 SpecificationsTech Info / DataCitationsDocumentationSpecificationsFormulationSupplied lyophilized in phosphate-buffered saline (PBS) containing 5% trehalose prior to lyophilizationAntigenHuman interferon betaIsotypeIgG1Host SpeciesMousePurityDetermined by protein A or G affinity chromatographyBioactivityMeasured by neutraliziation of interferon in antiviral assay; ND50 on human (HeLa/EMCV) cells is ~7-21 μg/ml in the presence of 10 ng/ml of human IFN-beta.SpecificityNeutralizes human IFN-beta; does not neutralize human IFN-alpha or gammaStorageFor retention of full activity store at -20°C or below and avoid repeated freeze/thaw cyclesAntigen SynonymsHuman Beta Interferon, Human IFNB, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN BCloneMMHB-2 Tech Info / Data Recommended Applications:NeutralizationDirect ELISA: 0.5 - 1.0 μg/mlWestern Blot: 1 - 2 μg/ml Citations1 Citation:Delaloye, Julie, et al. (2015). Interleukin-1- and Type I Interferon-Dependent Enhanced Immunogenicity of an NYVAC-HIV-1 Env-Gag-Pol-Nef Vaccine Vector with Dual Deletions of Type I and Type II Interferon-Binding Proteins. JVI, 14 pgs. PMID: 25609807. (link) Documentation 21410-1 Certificate of Analysis (CoA) SDS Neutralization of Hu-IFN-β To measure the ability of the antibody to neutralize the bioactivity of the human interferon beta on HeLa cells, Hu-IFN-ß was added to various concentrations of the antibody. The antigen-antibody mixture was added to confluent cultures of the HeLa cells in a 96-well plate. The assay mixture in a total volume of 100 µl, containing antibody at the concentrations as indicated, Hu-IFN-ß at 10 ng/ml, was incubated at 37ºC for 20-24 hours in a humidified CO2 incubator. At the end of this incubation period, medium was aspirated from all wells and an appropriate titrated amount of the EMCV in pre-warmed culture medium was added to each test well. After another 20-24 hour incubation, the cells were fixed, stained and scored for cytopathic effect by measurement of optical densities in a microplate reader at 540 nm. The ND50 of the antibody is approximately 7-21 µg/ml.