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PBL/Anti-Human IFN Beta Antibody, Clone MMHB-12 (MAb)/50 μg/21450-1
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Anti-Human IFN Beta Antibody, Clone MMHB-12 (MAb)Home / Products / Antibodies / Monoclonal Antibodies (MAbs) / Anti-Human IFN MAbs / Anti-Human IFN Beta Antibody, Clone MMHB-12 (MAb)Product Features:Mouse monoclonal antibody against Hu IFN Beta (Clone MMHB-12)Suitable for use in Direct Binding ELISA OrderingCatalog No. Pack Size Price Quantity 21450-1 50 μg$195.00 SpecificationsTech Info / DataCitationsDocumentationSpecificationsFormulationSupplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)AntigenHuman interferon betaIsotypeIgG1Host SpeciesMousePurity> 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μgSpecificityBinds to human IFN-beta with high affinityStorageFor retention of full activity store at -20°C or below and avoid repeated freeze/thaw cyclesAntigen SynonymsIFN BetaCloneMMHB-12 Tech Info / DataTested Application:Direct Binding ELISA Citations3 Citations:Kalb, Madeleine, et al. (2012). TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-α-mediated antitumor reactivity. Journal of Immunology, 10 pgs. PMID: 22231699. (link)Inglefield, JR, et al. (2008). TLR7 agonist 852A inhibition of tumor cell proliferation is dependent on plasmacytoid dendritic cells and type I IFN. Journal of Interferon & Cytokine Research. PMID: 18439103. (link)Moll, Herwig, et al. (2008). Neutralizing Type I Interferon Antibodies trigger an Interferon-Like Response in Endothelial Cells1. Journal of Immunology, 16 pgs. PMID: 18390705. (link) Documentation 21450-1 Certificate of Analysis (CoA) SDS Representative Binding Curve Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 0.1% BSA/PBS (square) in a Direct Binding ELISA. Medium-binding polystyrene plates were coated with either 1 µg/ml human interferon beta 1a or 0.1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3,3",5,5"-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a H2SO4/HCl solution.

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