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MenuProductsProduct CategoryAntibodiesAssay Kits NewES Cell LinesPureStem® Progenitor CellsECM Substrates and ComponentsGrowth FactorsSmall MoleculesPopular BrandsES Cell International™PureStem®PromotionsNew ProductsVacuNet™ Assay KitsApplicationsStem Cell ReprogrammingStem Cell DifferentiationStem Cell AnalysisStem Cell CultureAbout UsOur StoryContact UsOur VisionLicensingPress RoomOur TechnologyCareersHow to OrderContact UsProductsProduct CategoryAntibodiesAssay Kits NewES Cell LinesPureStem® Progenitor CellsECM Substrates and ComponentsGrowth FactorsSmall MoleculesPopular BrandsES Cell International™PureStem®PromotionsNew ProductsVacuNet™ Assay KitsApplicationsStem Cell ReprogrammingStem Cell DifferentiationStem Cell AnalysisStem Cell CultureAbout UsOur StoryContact UsOur VisionLicensingPress RoomOur TechnologyCareersHow to OrderContact Us .stores-top-nav {background:#1d2841;height: 30px;padding-top: 13px;}.stores-top-nav a {color: white;padding: 14px 20px 13px;transition: .3s ease;}.stores-top-nav a:hover {background: rgba(250,250,250,0.1);}.stores-top-nav a.active {background:#255aa7;}Home/ Products/ Product Category/ Antibodies/ BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µLZoom BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µLBe the first to review this productDetects SSEA-1 expression in live, unfixed pluripotent stem cell populations by immunofluorescent staining.SKU: ST11006$270.00Add to CartQty:Share Order Offline:T: 877.636.4978 E: orders@esibio.comF: 510.648.3255 DescriptionDetailsReviewsTagsDescriptionDetailsBioLite™ surface markers allow for direct immunofluorescent staining of live, unfixed pluripotent stem cell populations. The antibodies undergo meticulous application testing and validation to ensure they are sterile, non-toxic, contain no sodium azide, and are free of culture pathogens. Colonies stained with BioLite antibodies are able to continue in culture with no adverse effect on proliferation and differentiation potential when compared to untreated cells.Background Stage-specific embryonic antigen-1 (SSEA-1) is a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma (EC), murine embryonic stem (ES), and murine and human germ (EG) cells. No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.DetailsDetails Concentration0.5 mg/mL Species ReactivityHuman, Mouse HostMouse Monoclonal CloneMC-480 IsotypeIgM, κ ImmunogenF9 tetracarcinoma stem cells (X-irradiated) Formulation0.2 μm filter sterilized phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is Storage and StabilityStore at 2-8°C protected from light. Stable for 6 months from date of receipt when stored as directed. The BioLite format contains no preservative and therefore must be handled under aseptic conditions. Applications Tested Immunofluorescence (IF) on live, unfixed mouse ES cells Recommended DilutionsImmunofluorescence 1:100 Flow Cytometry 1:100 It is recommended that the antibody be titrated for optimal performance for each application. Alternative NamesCD15, Stage-specific embryonic antigen 1 ReferencesBrambrink, T., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159. PMID: 18371436Draper, J.S., et al. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258. PMID: 12033729 Technical Documents ST11006 Technical Data Sheet Protocol: Immunofluorescent Staining of Live CellsReviewsWrite Your Own ReviewYou"re reviewing: BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL*Nickname*Summary of Your Review*ReviewSubmit ReviewTagsTagsOther people marked this product with these tags:cell (3) live (1) staining (1)Add Your Tags:Add TagsUse spaces to separate tags. Use single quotes (") for phrases. 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