DescriptionDetailsReviewsTagsDescriptionDetailsThis antibody recognizes the stage-specific embryonic antigen-3 (SSEA-3) that is expressed upon the surface of human teratocarcinoma stem (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES/iPS cells may be accompanied by an increase in SSEA-3 expression.Sample DataFigure A: FC analysis on H1 human ES cells at a 1:100 dilution.Red histogram represents SSEA-3 Antibody and open histogram represents isotype control. A PE-conjugated anti-Rat IgM was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with SSEA-3 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).DetailsDetails Concentration0.5 mg/mL Species ReactivityHuman, Mouse HostRat Monoclonal CloneMC-631 IsotypeIgM Immunogen4-8 cell stage mouse embryos FormulationAqueous buffer, 0.09% sodium azide. Storage and StabilityStore at 2-8°C. Stable for 6 months from date of receipt when stored as directed. Applications Tested Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF) Recommended DilutionsFlow Cytometry 1:100Immunocytochemistry/Immunofluorescence 1:100It is recommended that the antibody be titrated for optimal performance for each application. Alternative NamesStage-specific embryonic antigen 3 ReferencesBrimble, S.N., et al. (2007) The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Stem Cells 25: 54-62. PMID: 17008424Henderson, J.K., et al. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337. PMID: 12110702Kannagi, R., et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J 2: 2355-2361. PMID: 6141938 Technical Documents ST11014 Technical Data SheetReviewsWrite Your Own ReviewYou"re reviewing: SSEA-3 anti-Human/Mouse Antibody, 100 µL*Nickname*Summary of Your Review*ReviewSubmit ReviewTagsTagsOther people marked this product with these tags:ICC (5) flow (5) SSEA3 (1) hES (14) human embryonic stem cell (4) IgM (2)Add Your Tags:Add TagsUse spaces to separate tags. Use single quotes (") for phrases.