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GloboZymes/PAK Detection Kit/GLO152-01

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GLO152-01

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PAK Detection KitGloboZymes PAK Detection Kit includes a 200 μg aliquot of affinity-purified Anti-PAK and a sample of highly purified PAK1 . The PAK1 aliquot is provided in SDS sample buffer ready for use in five applications as positive control in Western blots.The antibody was produced in rabbits to a synthetic peptide corresponding to residues 484-502 of human PAK1. The polyclonal antibody is useful for Western blotting. For example, it is used at 1:1000 dilution with 30 μg HEK293 cell extracts . In addition to PAK1, the antibody displays reactivity with PAK2.Synonyms: PAK, p21-Activated Protein Kinase, p65PAK, p68PAK Western Blot Detection KitFigure: An extract of HEK293 cells was subjected to Western blotting with GloboZymes affinity-purified Anti-PAK polyclonal antibody. The arrow denotes the position of PAK65 (65 kDa).Background: The p21-GTPase-activated protein kinases (PAK’s) are a family of enzymes that regulate numerous processes. These processes include cell morphology and cytoskeleton organization, cell motility, apoptosis, neuronal functions, cell cycle progression, viral replication and other processes. PAK’s interact with several important regulatory proteins including protein phosphatase 2A (PP2A), ribosomal protein S6 kinase, Mixed Lineage Kinase (MLK2), and the SH3-SH2 adapter Nck. Physiological targets of PAK include p38 MAP kinase, JNK, cRaf-1 and Synapsin I. The catalytic domain of PAK is produced in apoptotic cells via a caspase-dependent pathway. Unlike the native enzymes, the catalytic domain does not require Rac or Cdc42 to undergo autophosphorylation and activation.References: Nature 367: 40; J Biol Chem 270: 21695

GloboZymesDEK蛋白

来源：在大肠杆菌中生产的带有GST标签的重组人肾脏。

纯度：通过SDS-PAGE＞ 90％，表观先生〜70-kDa。

提供：在50μl50 mM Tris-HCl pH 7.0缓冲液中1μg，该缓冲液包含14 mMβ-巯基乙醇，1 mM苯甲idine，0.1 mM苯基甲磺酰氟，1 mM EDTA和10％甘油。将制剂分装在-70°C下。避免反复融化。

别名：DEK-GST癌蛋白

背景：DEK是一种明显的Mr〜43 kDa的核磷酸蛋白，由于急性髓性白血病和骨髓增生异常综合症的一部分发生复发性（6; 9）染色体易位，与核孔蛋白CAN融合。DEK还可以在青少年类风湿关节炎和其他风湿性疾病中充当自身免疫抗原。DEK与转录共抑制子Human Daxx，SRm160剪接共激活子和HIV-2增强子相关。它通过将约束的正超螺旋引入无蛋白和染色质相关的DNA中来改变DNA的拓扑。DEK与I2PP2A / SET具有某些结构相似性，在急性未分化白血病中也与CAN融合蛋白出现。但是，DEK确实会直接影响PP2A的活性。