OverviewData/SpecificationsLiterature/SupportHow To UseRelated ProductsOverviewOverview:Monoclonal antibody to delta-unsaturated glucuronic acid adjacent to N-acetylgalactosamine- 4-sulphate in the non-reducing terminal dissacharide “stub” of 4-sulphated chondroitin sulfate that is produced after chondroitinase digestion of chondroitin-4- sulphate glycosaminoglycan chains (chondroitinase ABC or ACII) or dermatan sulphate glycosaminoglycan chains (chondroitinase ABC or chondroitinase B).Related Terms/Symbols:Chondroitin-4-sulfateC-4-SDermatan-sulfateData/SpecificationsData/Specifications:Immunogen: Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABCdigestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).Clone: 2B6Isotype: IgGHost: MousePurity: Affinity purifiedSpecificity: Chondroitin-4-sulphate (C-4-S) after treatment with Chondroitinase ABC or ACII. Dermatansulphate (DS) neoepitope after treatment with Chondroitinase ABC or B.Form: liquidStorage: -20° CLiterature/SupportLiterature/Support/Publications:Antibody to Chondroitinase-generated C-4-S and DS neoepitope Insert (PDF, 338 KB)Joint Disease and Aggrecan (blog post)Caterson, B, Christner, JE, Baker, JR, Couchman, JR. (1985) Production and character- ization of monoclonal antibodies directed against connective tissue proteoglycans Fed Proc 44,386-393Couchman, J.R., Caterson, B., Christner, J.E. and Baker, J.R. (1984). Mapping by Monoclonal Antibody Detection of Glycosaminoglycans in Connective Tissues. Nature 307: 650-652Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B & Dent CM (2000). Catabolism of aggrecan, decorin and biglycan in tendon. Biochem J. 350: 181-188.Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem. 55(8):853-66.Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemisrty in extracellular matrix research. Methods 45: 10-21Stefan Milz, Frank Regner, Reinhard Putz, and Michael Benjamin. Expression of a Wide Range of Extracellular Matrix Molecules in the Tendon and Trochlea of the Human Superior Oblique MuscleInvestigative Ophthalmology and Visual Science. 2002;43:1330-1334Hedlund, H, Hedbom, E, Heinegard, D, Mengarelli-Widholm, S, Reinholt, FP, Svensson, O. (1999) Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage J Biol Chem 274,5777-5781Caterson, B, Christner, JE, Baker, JR, Couchman, JR. (1985) Production and character- ization of monoclonal antibodies directed against connective tissue proteoglycans Fed Proc 44,386-393Couchman, J.R., Caterson, B., Christner, J.E. and Baker, J.R. (1984). Mapping by Monoclonal Antibody Detection of Glycosaminoglycans in Connective Tissues. Nature 307: 650-652Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B & Dent CM (2000). Catabolism of aggrecan, decorin and biglycan in tendon. Biochem J. 350: 181-188.Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem. 55(8):853-66.Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemisrty in extracellular matrix research. Methods 45: 10-21Stefan Milz, Frank Regner, Reinhard Putz, and Michael Benjamin. Expression of a Wide Range of Extracellular Matrix Molecules in the Tendon and Trochlea of the Human Superior Oblique MuscleInvestigative Ophthalmology and Visual Science. 2002;43:1330-1334Hedlund, H, Hedbom, E, Heinegard, D, Mengarelli-Widholm, S, Reinholt, FP, Svensson, O. (1999) Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage J Biol Chem 274,5777-5781How To UseHow To Use:ApplicationsELISAWestern BlotImmunohistochemistryTechnical NotesPretreatment with Chondroitinase required. 1x10-3 U per 10 mg GAG at 37 °C for 1 hour.Related ProductsRelated Products:Aggrecan Antibody, C-terminal neoepitope NITEGEAggrecan Antibody, N-terminal neoepitope ARGAggrecan Antibody, N-terminal neoepitope DIPENAggrecan Antibody, N-terminal neoepitope FFGVAggrecan IGD AntibodyReverse Related Products:Chondroitin Sulphate Neoepitope Antibody