OverviewData/SpecificationsLiterature/SupportHow To UseRelated ProductsOverviewOverview:Type I collagen is the most abundant form of collagen in the human body and is synthesized mainly by fibroblasts, osteoblasts, odontoblasts and chondroblasts.It is located in the extracellular matrix of many tissues of the body including cartilage, bone, tendon, skin and the sclera of the eye.Type I collagen is composed of two pro-α1(I) chains, produced from the COL1A1 gene, and one pro-α2(I) chain, produced from the COL1A2 gene.Mutations in the genes that produce collagen type I are responsible for causing various health conditions including Ehlers-Danlos syndrome, osteogenesis imperfecta, osteoporosis and Caffey disease.Data/SpecificationsData/Specifications:Format: IgG fraction, freeze-dried (0.5 ml). Reconstitute with 0.5 mldi H2O and store aliquots at -20°C.Specificity (% at 1:500 RIA dilution):Mouse Collagen, Type I : 100%Mouse Collagen, Types II, IV : < 0.1%Mouse collagen type III: < 1.0%Human, chicken, rat Collagen Type I: < 0.1%Literature/SupportLiterature/Support/Publications:Anti-Mouse Collagen Type I Antibody Insert (PDF, 283 KB)References:Saraswati, S., Lietman, C. D., Li, B., Mathew, S., Zent, R., & Young, P. P. (2020). Small proline?rich repeat 3 is a novel coordinator of PDGFR? and integrin ?1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts. The FASEB Journal.Saraswati, S., Lietman, C. D., Li, B., Mathew, S., Zent, R., & Young, P. P. (2020). Small proline-rich repeat 3 is a novel coordinator of PDGFRβand integrinβ1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts. The FASEB Journal.Saraswati, S., Marrow, S. M., Watch, L. A., & Young, P. P. (2019). Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing.Nature Communications,10(1), 1-16.Nawaito, S. A., Sahadevan, P., Sahmi, F., Gaestel, M., Calderone, A., & Allen, B. G. (2019).Transcript levels for extracellular matrix proteins are altered in MK5-deficient cardiac ventricular fibroblasts.Journal of Molecular and Cellular Cardiology,132, 164-177.Pesevski, Z., Kvasilova, A., Stopkova, T., Nanka, O., Drobna Krejci, E., Buffinton, C., ... & Sedmera, D. (2018). Endocardial fibroelastosis is secondary to hemodynamic alterations in the chick embryonic model of hypoplastic left heart syndrome.Developmental Dynamics,247(3), 509-520.Surinkaew, S., Aflaki, M., Takawale, A., Chen, Y., Qi, X. Y., Gillis, M. A., ... & Nattel, S. (2018). Exchange protein activated by cyclic-adenosine monophosphate (Epac) regulates atrial fibroblast function and controls cardiac remodelling.Cardiovascular research,115(1), 94-106.Viquez, O. M., Yazlovitskaya, E. M., Tu, T., Mernaugh, G., Secades, P., McKee, K. K., ... & Gewin, L. C. (2017). Integrin alpha6 maintains the structural integrity of the kidney collecting system.Matrix Biology,57, 244-257.Feng, Y., Wang, S., Zhang, Y., & Xiao, H. (2017). Metformin attenuates renal fibrosis in both AMPK α2‐dependent and independent manners.Clinical and Experimental Pharmacology and Physiology,44(6), 648-655.Chen, X., & Thibeault, S. L. (2016). Cell–cell interaction between vocal fold fibroblasts and bone marrow mesenchymal stromal cells in three‐dimensional hyaluronan hydrogel.Journal of tissue engineering and regenerative medicine,10(5), 437-446.Dupuis, L. E., Doucette, L., Rice, A. K., Lancaster, A. E., Berger, M. G., Chakravarti, S., & Kern, C. B. (2016). Development of myotendinous‐like junctions that anchor cardiac valves requires fibromodulin and lumican.Developmental Dynamics,245(10), 1029-1042.Seet, L. F., Toh, L. Z., Finger, S. N., Chu, S. W., Stefanovic, B., & Wong, T. T. (2016). Valproic acid suppresses collagen by selective regulation of Smads in conjunctival fibrosis.Journal of Molecular Medicine,94(3), 321-334.Pankova, D., Chen, Y., Terajima, M., Schliekelman, M. J., Baird, B. N., Fahrenholtz, M., ...& Ahn, Y. H. (2016). Cancer-associated fibroblasts induce a collagen cross-link switch in tumor stroma.Molecular Cancer Research,14(3), 287-295.Neelisetty, S., Alford, C., Reynolds, K., Woodbury, L., Nlandu-khodo, S., Yang, H., ... & Gewin, L. (2015). Renal fibrosis is not reduced by blocking transforming growth factor-β signaling in matrix-producing interstitial cells.Kidney international,88(3), 503-514.Wang, H., Chen, X., Su, Y., Paueksakon, P., Hu, W., Zhang, M. Z., ... & Pozzi, A. (2015). p47phox contributes to albuminuria and kidney fibrosis in mice.Kidney international,87(5), 948-962.Singh, S. P., Tao, S., Fields, T. A., Webb, S., Harris, R. C., & Rao, R. (2015). Glycogen synthase kinase-3 inhibition attenuates fibroblast activation and development of fibrosis following renal ischemia-reperfusion in mice.Disease models & mechanisms,8(8), 931-940.Trombetta‐eSilva, J., Rosset, E. A., Hepfer, R. G., Wright, G. J., Baicu, C., Yao, H., & Bradshaw, A. D. (2015). Decreased Mechanical Strength and Collagen Content in SPARC‐Null Periodontal Ligament Is Reversed by Inhibition of Transglutaminase Activity.Journal of bone and mineral research,30(10), 1914-1924.Zhu, M., Tao, J., Vasievich, M. P., Wei, W., Zhu, G., Khoriaty, R. N., & Zhang, B. (2015). Neural tube opening and abnormal extraembryonic membrane development in SEC23A deficient mice.Scientific reports,5, 15471.Manley Jr, E., Perosky, J. E., Khoury, B. M., Reddy, A. B., Kozloff, K. M., & Alford, A. I. (2015). Thrombospondin-2 deficiency in growing mice alters bone collagen ultrastructure and leads to a brittle bone phenotype.Journal of Applied Physiology,119(8), 872-881.Sochman, J., Peregrin, J. H., Pavcnik, D., Uchida, B. T., Timmermans, H. A., Sedmera, D., ... & Rosch, J. (2014). Reverse endoventricular artificial obturator in tricuspid valve position. Experimental feasibility research study.Physiological research,63(2), 157.Bohuslavova, R., Kolar, F., Sedmera, D., Skvorova, L., Papousek, F., Neckar, J., & Pavlinkova, G. (2014). Partial deficiency of HIF-1α stimulates pathological cardiac changes in streptozotocin-induced diabetic mice.BMC endocrine disorders,14(1), 11.Chen, X., Wang, H., Liao, H. J., Hu, W., Gewin, L., Mernaugh, G., ... & Fässler, R. (2014). Integrin-mediated type II TGF-β receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling.The Journal of clinical investigation,124(8), 3295-3310.Zimmerman, K. A., Graham, L. V., Pallero, M. A., & Murphy-Ullrich, J. E. (2013).Calreticulin (CRT) regulates Transforming Growth Factor-β (TGF-β) stimulated extracellular matrix production.Journal of Biological Chemistry, jbc-M112.Rosa, R. G., Akgul, Y., Joazeiro, P. P., & Mahendroo, M. (2012). Changes of large molecular weight hyaluronan and versican in the mouse pubic symphysis through pregnancy.Biology of reproduction,86(2).Baicu, C. F., Zhang, Y., Van Laer, A. O., Renaud, L., Zile, M. R., & Bradshaw, A. D. (2012). Effects of the absence of procollagen C-endopeptidase enhancer-2 on myocardial collagen accumulation in chronic pressure overload.American Journal of Physiology-Heart and Circulatory Physiology,303(2), H234.Dawson, K., Wu, C. T., Qi, X. Y., & Nattel, S. (2012). Congestive heart failure effects on atrial fibroblast phenotype: differences between freshly-isolated and cultured cells.PLoS One,7(12), e52032.Chen, J., Chen, J. K., Nagai, K., Plieth, D., Tan, M., Lee, T. C., ...& Harris, R. C. (2012). EGFR signaling promotes TGFβ-dependent renal fibrosis.Journal of the American Society of Nephrology,23(2), 215-224.Dagher, P. C., Mai, E. M., Hato, T., Lee, S. Y., Anderson, M. D., Karozos, S. C., ...& Sutton, T. A. (2011). The p53 inhibitor pifithrin-α can stimulate fibrosis in a rat model of ischemic acute kidney injury.American Journal of Physiology-Renal Physiology,302(2), F284-F291.References/Citations:How the anti-mouse collagen type I antibody was used: Cervical Softening During Pregnancy: Regulated Changes in Collagen Cross-Linking and Composition of Matricellular Proteins in the Mouse.Meredith L. Akins, Katherine Luby-Phelps, Ruud A. Bank, and Mala MahendrooBiol Reprod,May 2011; 84: 1053 - 1062The Rabbit Anti-Mouse Collagen Type I Polyclonal Antibody was used in immunoblot studies. Collagen from cervical tissuewas extracted with 7 M Urea, 0.1M sodium phosphate with 1% protease inhibitor overnight at 4 °C. The protein concentration was determined using a Bradford protein assay.Ten micrograms of protein were loaded on a 4%–20% Tris-HCl polyacrylamide gel and electrophoresed at 100 V. After overnight transfer to nitrocellulose membrane and Ponceu S staining to assess equal loading of protein, immunoblotting was performed using rabbit polyclonal anti-mouse collagen I.Intracellular Calreticulin Regulates Multiple Steps in Fibrillar Collagen Expression, Trafficking, and Processing into the Extracellular Matrix Lauren Van Duyn Graham, et al. J. Biol. Chem., Mar 2010; 285: 7067 - 7078.The Rabbit Anti-Mouse Collagen Type I Polyclonal Antibody was used in immunoblot, immunocytochemistry and immunoprecipitation studies of wild-type mouse embryonic fibroblasts (MEFs) and Calreticulin(-/-) MEFs.In immunoblot analysis, the antibody recognized the three forms of the alpha(I) chain of collagen type I: the unprocessed form with the N- and C-propeptides, the N-propeptide cleaved; and the fully processed alpha(I) band.In immunocytochemistry, the collagen type Iprimary antibody was used at 1:200 dilution for 2 hrs, followed by the secondary antibody AlexaFluor 488 goat anti-rabbit IgG used at 1:300 for 1 hr.In immunoprecipitation studies, beads were incubated with 6 ug of the antibody.Sirt1 activation protects the mouse renal medulla from oxidative injury. He W, et al. J Clin Invest. 2010 Apr; 120(4):1056-68.Immunoblot analysis to measure the expression profile of type I collagen in contralateral and obstructed kidneys obtained from mice.Type XIV collagen regulates fibrillogenesis: premature collagen fibril growth and tissue dysfunction in null mice. Ansorge HL, et al. J Biol Chem. Mar 2009; 284(13): 8427-38.Immunohistochemistry analysis to characterize the expression profile of type I collagen in flexor digitorum longus (FDL) tendons that were dissected from mice.Tissues were embedded and snap-frozen in OCT medium, followed by sectioning at 6 micrometers thick.The primary antibody was used at a 1:1000 dilution.SPARC Regulates Processing of Procollagen I and Collagen Fibrillogenesis in Dermal Fibroblasts Tyler J. Rentz et al., J. Biol. Chem., Jul 2007; 282: 22062 - 22071.Immunoblotand immunocytochemistry (1:200 dilution) analysis to measure and characterize the expression profile of type I collagen in cultured primary dermal fibroblasts that were isolated from mice. The Calreticulin-Binding Sequence of Thrombospondin 1 Regulates Collagen Expression and Organization During Tissue RemodelingMariya T. Sweetwyne, Manuel A. Pallero, Ailing Lu, Lauren Van Duyn Graham, and Joanne E. Murphy-UllrichAm. J. Pathol., Oct 2010; 177: 1710 - 1724.Imunoblot analysis of cell lysates How To UseHow To Use:For immunostaining of extra or intracellular components inlight microscopy and the quantitation of Collagen Type I inbiological fluids or in culture supernatants.ApplicationsIFAIHC(p)(SP) RIAELISAIB/WBWorking DilutionsIndirect IFA (with fluoresceine anti-rabbit IgG conjugate): >1:80 on frozen mouse tissues (liver, skin...).IHC(p): >1:500 on fixed, paraffin-embedded mouse tissuesELISA: >1:200 (OD >500)Related ProductsRelated Products:Collagen Type I Antibody, anti-mouseCollagen Type I Antibody, anti-ratReverse Related Products:Collagen Type I (Atelocollagen), BovineCollagen Type I (Atelocollagen), MouseCollagen Type I (Atelocollagen), RatCollagen Type I and III, BovineCollagen Type I and III, canineCollagen Type I and III, mouseCollagen Type I and III, PorcineCollagen Type I and III, RatCollagen Type I Antibody, anti-mouseCollagen Type I Antibody, anti-ratCollagen Type I, BovineCollagen Type I, GoatCollagen Type I, MouseCollagen Type I, RatCollagen Type II Antibody, anti-humanCollagen Type III (Atelocollagen) Bovine Collagen Type III, BovineCollagen Type IV Antibody, anti-rat