OverviewData/SpecificationsLiterature/SupportHow To UseRelated ProductsOverviewOverview:Proteoglycans are categorized depending upon the nature of their glycosaminoglycan chains (chondroitin sulfate, dermatan sulfate, heparan sulphate and keratan sulfate) as well as characterized by size.Aggrecan is a large aggregating proteoglycan of articular cartilage. It is also found in aorta tissue, discs, tendons and in the perineuronal net. It is responsible for hydrating cartilage, giving it compressibility and resilience during joint loading, thereby playing a major role in the normal function of cartilage. Depletion of glycosaminoglycan-bearing aggrecan fragments is one of the earliest events in cartilage destruction.Data/SpecificationsData/Specifications:Immunogen:Synthetic peptide: EPEEPFTFAPEIClone:6B4Host: MouseMyeloma: x63-Ag8.653Isotype: IgG1Light Chain type: kappaSpecificity:Recognizes the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobulardomain (IGD) of human and bovine aggrecan.Cross-reactivity: This antibody cross-reacts with human and bovinePurity: Affinity purified on protein GForm: Liquid, 1 mL/vialConcentration: 0.1 mg/mLStorage: -20° CLiterature/SupportLiterature/Support/Publications:Aggrecan IGD Antibody Insert (PDF, 334 KB)Joint Disease and Aggrecan (blog post)How To UseHow To Use:ApplicationsELISA: Use at an assay dependant dilution.IHC-P: Use at an assay dependant dilution.IHC-Fr: Use at an assay dependant dilution.WB: Use at a dilution of 1/100.Technical NotesOptimal dilutions/concentrations should be determined by the end user. This antibodyshould work in IHC on formalin or paraformaldehyde-fixed paraffin embedded sections aswell as either alcohol-fixed frozen sections or un-fixed snap-frozen sections.Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAGof non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.Related ProductsRelated Products:Aggrecan Antibody, C-terminal neoepitope NITEGEAggrecan Antibody, N-terminal neoepitope ARGAggrecan Antibody, N-terminal neoepitope DIPENAggrecan Antibody, N-terminal neoepitope FFGVReverse Related Products:Aggrecanase Activity ELISAAggrecanase Activity, Sensitive ELISACartilage Link Protein, AntibodyChondroitin Sulphate Neoepitope AntibodyChondroitinase generated C-4-S & DS AntibodyKeratan Sulfate AntibodyKeratocan AntibodyLubricin Antibody, BovineLubricin Antibody, Native BovineMMP-13 (activated) ELISA