OverviewData/SpecificationsLiterature/SupportHow To UseRelated ProductsOverviewOverview:During differentiation of neural precursor cells, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology.Data/SpecificationsData/Specifications:Immunogen:ARGSV synthetic peptide conjugate.Clone: BC-3Host: MouseMyeloma: x63-Ag8.653Isotype: IgG1Light Chain type: kappaSpecificity:Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognize the sequence ARG if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognize the aggrecanase generated neoepitope ARG.Cross-reactivity:human, rat, cat, cow, dog, guinea pig, horse, pig, rabbit and sheep.Purity: Affinity purified on protein GForm: Liquid, 1 mL/vialConcentration: 0.1 mg/mLStorage: -20° CLiterature/SupportLiterature/Support/Publications:Aggrecan antibody, clone BC-3 Insert (PDF, 335 KB)Joint Disease and Aggrecan (Blog Post)How To UseHow To Use:ApplicationsWestern Blot -Suggested dilution: 1:100.Detects a variety of epitopes between 50 and 250 kDaELISAIHC with frozen sections or formalin/PFA-fixed paraffin embedded sectionsTechnical NotesSamples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 UnitsKeratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG of nondeglycosylatedaggrecan for optimal epitope recognition. Related ProductsRelated Products:Aggrecan Antibody, N-terminal neoepitope DIPENAggrecan Antibody, N-terminal neoepitope FFGVReverse Related Products:Aggrecan Antibody, C-terminal neoepitope NITEGEAggrecan Antibody, N-terminal neoepitope DIPENAggrecan Antibody, N-terminal neoepitope FFGVAggrecan IGD AntibodyAggrecanase Activity ELISAAggrecanase Activity, Sensitive ELISACartilage Link Protein, AntibodyChondroitin Sulphate Neoepitope AntibodyChondroitinase generated C-4-S & DS AntibodyHyaluronic Acid Binding Region, AntibodyKeratan Sulfate AntibodyKeratocan AntibodyLubricin Antibody, BovineLubricin Antibody, Native BovineMMP-13 (activated) ELISA