OverviewData/SpecificationsLiterature/SupportHow To UseRelated ProductsOverviewOverview:Type I collagen is the most abundant form of collagen in the human body and is synthesized mainly by fibroblasts, osteoblasts, odontoblasts and chondroblasts.It is located in the extracellular matrix of many tissues of the body including cartilage, bone, tendon, skin and the sclera of the eye.Type I collagen is composed of two pro-α1(I) chains, produced from the COL1A1 gene, and one pro-α2(I) chain, produced from the COL1A2 gene.Mutations in the genes that produce collagen type I are responsible for causing various health conditions including Ehlers-Danlos syndrome, osteogenesis imperfecta, osteoporosis and Caffey disease.Data/SpecificationsData/Specifications:Format: IgG fraction, freeze-dried (0.5 ml). Reconstitute with 0.5 mldi H2O and store aliquots at -20°C.Specificity (% at 1:200 RIA dilution):Rat Collagen, Type I : 100%Rat Collagen, Types II, III, V : < 0.1%Human, mouse, chicken Collagen Type I: < 0.1%Rat elastin: <0.1%Literature/SupportLiterature/Support/Publications:Anti-Rat Collagen Type I Antibody Insert (PDF, 283 KB)References:Dagher, P. C., Hato, T., Mang, H. E., Plotkin, Z., Richardson, Q. V., Massad, M., ... & Sutton, T. A. (2016). Inhibition of toll-like receptor 4 signaling mitigates microvascular loss but not fibrosis in a model of ischemic acute kidney injury.International journal of molecular sciences,17(5), 647.Sutton, T. A., Hato, T., Mai, E., Yoshimoto, M., Kuehl, S., Anderson, M., ... & Dagher, P. C. (2013). p53 is renoprotective after ischemic kidney injury by reducing inflammation.Journal of the American Society of Nephrology,24(1), 113-124.Dagher, P. C., Mai, E. M., Hato, T., Lee, S. Y., Anderson, M. D., Karozos, S. C., ... & Sutton, T. A. (2011). The p53 inhibitor pifithrin-α can stimulate fibrosis in a rat model of ischemic acute kidney injury.American Journal of Physiology-Renal Physiology,302(2), F284-F291.How To UseHow To Use:For immunostaining of extra or intracellular components inlight microscopy and the quantitation of Collagen Type I inbiological fluids or in culture supernatants.ApplicationsIFAIHC(p)(SP) RIAELISAWorking DilutionsIndirect IFA (with fluoresceine anti-rabbit IgG conjugate): >1:80 on frozen rat tissues (liver, skin...).IHC(p): >1:500 on fixed, paraffin-embedded rat tissuesELISA: >1:200 (OD <500)Related ProductsRelated Products:Collagen Type I Antibody, anti-mouseCollagen Type II, Bovine, LyophilizedCollagen Type II, Bovine, SolubleCollagen Type II, Chicken, LyophilizedCollagen Type II, Chicken, SolubleCollagen Type II, Rat LathyriticCollagen Type II, Rat LathyriticCollagen Type II, Rat Pepsin DigestedCollagen Type II, Rat Pepsin-DigestedReverse Related Products:Collagen Type I (Atelocollagen), BovineCollagen Type I (Atelocollagen), MouseCollagen Type I (Atelocollagen), RatCollagen Type I and III, BovineCollagen Type I and III, canineCollagen Type I and III, mouseCollagen Type I and III, PorcineCollagen Type I and III, RatCollagen Type I Antibody, anti-mouseCollagen Type I, BovineCollagen Type I, GoatCollagen Type I, MouseCollagen Type I, RatCollagen Type II Antibody, anti-humanCollagen Type III (Atelocollagen) Bovine Collagen Type III, BovineCollagen Type IV Antibody, anti-rat