

提醒:代购产品,无质量问题不接受退换货,下单前请仔细核对信息。下单后请及时联系客服
核对商品价格,订单生效后再付款。
MD Biosciences/Cell Proliferation Kit/1000 Assays/409005
价格:
自营商城
解放采购
正品保障
及时交付
厂家直采
一站服务
货号:
品牌:
会员服务:
尊享会员价
贵宾专线
运费优惠
闪电退款
福利优惠
上门换新
友情提示
以上价格仅为参考,请联系客服询价。
免费咨询热线
4000-520-616
产品说明
OverviewData/SpecificationsLiterature/SupportHow It WorksOverviewOverview:The need for a reliable, sensitive and quantitative assay that would enable analysis of a large number of samples led to the development of methods, such as: use of radioactive thymidine to label DNA in live cells use of Brdu to label DNA in live cells (as a substitute for radioactive thymidine) The advantages of using MD Bioproducts Cell Proliferation Kit (XTT) can be summarized with the following attributes: easy-to-use: there is no requirement for additional reagents and/or the cell washing procedures speed: multiwell plates and an ELISA reader can be used for reading sensitivity: can be assayed even in low cell concentrations accuracy: dye absorbance is proportional to the number of cells in each well safety: there is no need for radioactive isotopes Data/SpecificationsData/Specifications: Kit Components XTT Reagent Activation Reagent Literature/SupportLiterature/Support/Publications:Cell Proliferation Kit Data Insert (PDF, 439 KB)Nagafuku, M., Okuyama, K., Onimaru, Y., Suzuki, A., Odagiri, Y., Yamashita, T. & Inokuchi, J. I. (2012). CD4 and CD8 T cells require different membrane gangliosides for activation. Proceedings of the National Academy of Sciences, 109(6), E336-E342.How It WorksHow It Works:The assay is based on the ability of metabolic active cells to reduce the tetrazolium salt XTT to orange colored compounds of formazan. The dye formed is water soluble and the dye intensity can be read at a given wavelength with a spectrophotometer. The intensity of the dye is proportional to the number of metabolic active cells. The use of multiwell plates and an ELISA reader enables testing a large number of samples and obtaining easy and rapid results. The test procedure includes cultivation of cells in a 96-well plate, adding the XTT reagent and incubation for 2-24 hours. During incubation an orange color is formed, the intensity of which can be measured with a spectrophotometer, in this instance with an ELISA reader. The greater the number of active cells in the well, the greater the activity of mitochondria enzymes, and the higher the concentration of the dye formed, which can then be measured and quantitated.
MD Biosciences 是针对药物和诊断资产开发的临床前,转化和临床阶段合同研究服务的供应商。通过AAALAC 认证,符合 GLP 的 CLIA 设施,在广泛的治疗领域提供服务和产品,包括炎性疾病,自身免疫,神经病学,肿瘤学,新陈代谢和疼痛。为了在每个专业领域提供最优质的产品和专业知识并优化研究,设有 4 个独立的部门:MD Biosciences炎症/免疫学服务、MD Biosciences神经病学发现服务、MD Biosciences组织病理学服务和MD Bioproducts。
新品排行榜
1
MD Biosciences/Mouse IgG1 Isotyp...
2
MD Biosciences/Cortisone Polyclo...
3
MD Biosciences/Collagen Type I A...
4
MD Biosciences/HtrA Serine Pepti...
5
MD Biosciences/Aggrecan Antibody...
6
MD Biosciences/Phenytoin Polyclo...
7
MD Biosciences/Aggrecan IGD Anti...
8
MD Biosciences/Aggrecan Antibody...
9
MD Biosciences/Collagen Type I (...
10
MD Biosciences/Aggrecanase Activ...
文章排行榜
1
硫酸软骨素新表位抗体,100 ug – MD Bioproducts
2
小鼠 IgG1 同型对照,生物素偶联抗体,0.5 毫克 – MD Bioproducts
3
大鼠 IgG1 同型对照,R-PE 缀合抗体,0.1 毫克 – MD Bioproducts
4
Lubricin 抗体,天然牛,100 ug – MD Bioproducts
5
小鼠 IgG1 同型对照,生物素偶联抗体,0.5 毫克 – MD Bioproducts
6
山羊抗小鼠 IgD,抗血清,1 mL – MD Bioproducts
7
III 型胶原蛋白,牛,10 毫克 – MD Bioproducts
8
T1/ST2 (IL-33 R) 单克隆抗体,不含叠氮化物,0.5 mL – MD Bioproducts
9
细胞增殖检测|MD Biosciences|md biosciences|试剂盒|MD...
10
脂多糖 (LPS),5.0 毫克 – MD Bioproducts
微信公众号

手机扫码,关注动态