SpecificationGene InfoPathwayDiseaseRelated ProductsList AllSpecificationProduct Description:This protein phosphorylation antibody pair set comes with two antibodies, one against the BRAF protein, and the other against the specific S601 phosphorylated site of BRAF for use in in situ Proximity Ligation Assay. See Publication Reference below.Reactivity:HumanQuality Control Testing:Dual recognition immunofluorescence result.Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.Supplied Product:Antibody pair set content: 1. Phospho-BRAF S601 rabbit polyclonal antibody (20 ul) With 0.09% sodium azide. 2. BRAF mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction:Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.Datasheet:DownloadPublication Reference1.In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J,Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.2.Direct observation of individual endogenous protein complexes in situ by proximity ligation.Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.3.Cytokine detection by antibody-based proximity ligation.Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, andFredriksson S. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.4.Protein detection using proximity-dependent DNA ligation assays.Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U. Nat Biotechnol. 2002 May;20(5):473-7.5.Highly specific detection of phosphorylated proteins by DuolinkMats Gullberg and Ann-Catrin Andersson Nature Methods 6. 2009ApplicationsIn situ Proximity Ligation Assay (Cell)Application ImageIn situ Proximity Ligation Assay (Cell)Gene InformationEntrez GeneID:673Gene Name:BRAFGene Alias:B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1Gene Description:v-raf murine sarcoma viral oncogene homolog B1Omim ID:115150, 164757, 211980Gene Ontology:HyperlinkGene Summary:This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeqOther Designations:94 kDa B-raf protein,B-Raf proto-oncogene serine/threonine-protein kinase (p94),Murine sarcoma viral (v-raf) oncogene homolog B1Gene PathwayAcute myeloid leukemiaBladder cancerChemokine signaling pathwayChronic myeloid leukemiaColorectal cancerEndometrial cancerErbB signaling pathwayFocal adhesionGliomaInsulin signaling pathwayLong-term depressionLong-term potentiationMAPK signaling pathwayMelanomamTOR signaling pathwayNatural killer cell mediated cytotoxicityNeurotrophin signaling pathwayNon-small cell lung cancerPancreatic cancerPathways in cancerProstate cancerRegulation of actin cytoskeletonRenal cell carcinomaThyroid cancerVascular smooth muscle contractionRelated Disease Abnormalities, Multiple Adenocarcinoma Adenocarcinoma, Follicular Adenocarcinoma, Mucinous Adenocarcinoma, Papillary Adenoma Adenoma, Oxyphilic Adrenal Cortex Neoplasms Alcoholism Articulation Disorders Astrocytoma Brain Neoplasms Breast cancer Breast Neoplasms Carcinoma Carcinoma, Basal Cell Carcinoma, Medullary Carcinoma, Non-Small-Cell Lung Carcinoma, Papillary ... see more Carcinoma, Papillary, Follicular Carcinoma, Squamous Cell Carcinoma, Transitional Cell Cell Transformation, Neoplastic Chromosomal Instability Chromosome Aberrations Cognition Cognition Disorders Colon cancer Colonic Neoplasms Colonic Polyps Colorectal Neoplasms Colorectal Neoplasms, Hereditary Nonpolyposis Common Bile Duct Neoplasms Craniofacial Abnormalities Cystadenocarcinoma, Mucinous Cystadenocarcinoma, Serous Cystadenoma, Serous Developmental Disabilities Dilatation, Pathologic Disease Progression Diseases in Twins Dyslexia, Acquired Ectodermal Dysplasia Endometrial Hyperplasia Endometrial Neoplasms Endometriosis Eosinophilia Esophageal Neoplasms Gastrointestinal Stromal Tumors Genetic Predisposition to Disease Glioma Goiter Goiter, Nodular Hashimoto Disease Head and Neck Neoplasms Hearing Heart Defects, Congenital Hyperpigmentation Hyperplasia Immunologic Deficiency Syndromes Language Disorders Liver Neoplasms Lung Neoplasms Lymphatic Metastasis Lymphoma, Non-Hodgkin Malignant melanoma Melanoma Memory Microsatellite Instability Motor Skills Nasopharyngeal Neoplasms Necrosis Neoplasm Invasiveness Neoplasm Metastasis Neoplasm Recurrence, Local Neoplasms Neoplasms, Germ Cell and Embryonal Neoplasms, Glandular and Epithelial Neoplasms, Multiple Primary Neoplasms, Radiation-Induced Nerve Sheath Neoplasms Neurofibromatosis Neurofibromatosis 1 Nevus Nevus, Pigmented Noonan Syndrome Obesity Ovarian cancer Ovarian Neoplasms Pancreatic cancer Pancreatic Neoplasms Papillomavirus Infections Precancerous Conditions Prostatic Neoplasms Ras oncogene Rectal Neoplasms Recurrence Salivary Gland Neoplasms Skin Neoplasms Stomach Neoplasms Syndrome Testicular Neoplasms Thyroid Diseases Thyroid Neoplasms Thyroid Nodule Thyroiditis, Autoimmune Tobacco Use Disorder Urinary Bladder Neoplasms ... hiddenRelated ProductDI0237 MAPK3 & BRAF Protein Protein Interaction Antibody 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