SpecificationGene InfoDiseaseRelated ProductsList AllSpecificationProduct Description:Mouse monoclonal antibody raised against partial recombinant human ALK.Immunogen:Recombinant protein corresponding to amino acids 1360-1460 of human ALK.Host:MouseTheoretical MW (kDa):80, 200Reactivity:HumanForm:LiquidPurification:Protein A/G purificationIsotype:IgG1, kappaRecommend Usage:Flow Cytometry (0.5-1 ug/106 cells)Immunofluorescence (0.5-1 ug/mL)Western Blotting (0.5-1 ug/mL)The optimal working dilution should be determined by the end user.Storage Buffer:In 10 mM PBS.Storage Instruction:Store at -20 to -80°C.Aliquot to avoid repeated freezing and thawing.Datasheet:DownloadPublication Reference1.Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1.Pulford K, Lamant L, Morris SW, Butler LH, Wood KM, Stroud D, Delsol G, Mason DY.Blood. 1997 Feb 15;89(4):1394-404.ApplicationsWestern BlotWestern Blot analysis of Lane 1: recombinant protein and Lane 2: HepG2 cell lysate with ALK monoclonal antibody, clone ALK/1503 (Cat # MAB14792).ImmunofluorescenceFlow CytometryApplication ImageWestern BlotenlargeImmunofluorescenceFlow CytometryGene InformationEntrez GeneID:238Protein Accession#:Q9UM73Gene Name:ALKGene Alias:CD246,Ki-1,TFG/ALKGene Description:anaplastic lymphoma receptor tyrosine kinaseOmim ID:105590Gene Ontology:HyperlinkGene Summary:The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3" half of ALK, derived from chromosome 2, is fused to the 5" portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeqOther Designations:ALK tyrosine kinase receptor,CD246 antigen,anaplastic lymphoma kinase (Ki-1),anaplastic lymphoma kinase Ki-1Related Disease Adenocarcinoma Carcinoma, Non-Small-Cell Lung Genetic Predisposition to Disease Kidney Failure, Chronic Lung Neoplasms Multiple Sclerosis Schizophrenia Schizophrenia Tobacco Use DisorderRelated ProductCS0001 ALK Split CISH ProbeFG0036 ALK/CEN2p FISH ProbeFP0014 mutaFISH™ EML4wt ALKwt RNA ProbesFS0001 ALK Split FISH ProbeFS0047 ALK Split FISH ProbeFT0001 EML4/ALK DY Translocation FISH ProbeFT0009 EML4/ALK Translocation FISH ProbeFT0019 VCL/ALK DY Translocation FISH ProbeH00000238-A01 ALK polyclonal antibody (A01)H00000238-B01P ALK purified MaxPab mouse polyclonal antibody (B01P)H00000238-H01 ALK (Human) Recombinant ProteinH00000238-K ALK rabbit monoclonal antibodyH00000238-M01 ALK monoclonal antibody (M01), clone 4D10H00000238-M02 ALK monoclonal antibody (M02), clone 4F6H00000238-M03 ALK monoclonal antibody (M03), clone 3E12H00000238-M04 ALK monoclonal antibody (M04), clone 4E5H00000238-M05 ALK monoclonal antibody (M05), clone 3A3H00000238-M06 ALK monoclonal antibody (M06), clone 2A5H00000238-Q01 ALK (Human) Recombinant Protein (Q01)KA2568 ALK (Human) Cell-Based ELISA KitMAB14536 ALK monoclonal antibody, clone ALK/1503MAB17851 ALK monoclonal antibody, clone 7A11A4MAB17856 ALK monoclonal antibody, clone 8E8D3MAB20989 ALK monoclonal antibody, clone ALK/1504MAB20990 ALK monoclonal antibody, clone ALK/1504MAB21994 ALK monoclonal antibody, clone RM361P4644 ALK (Human) Recombinant ProteinP5736 ALK (C1156Y) (Human) Recombinant ProteinP5741 ALK (F1174L) (Human) Recombinant ProteinP5742 ALK (L1196M) (Human) Recombinant ProteinP5747 ALK (R1275Q) (Human) Recombinant ProteinP5765 ALK (Human) Recombinant ProteinP5782 NPM1-ALK (Human) Recombinant ProteinP5783 EML4-ALK (Human) Recombinant ProteinP6462 ALK (G1202R) (Human) Recombinant ProteinP6463 ALK (G1269A) (Human) Recombinant ProteinP6464 ALK T1151_L1152insT (Human) Recombinant ProteinPAB17019 ALK (phospho Y1586) polyclonal antibodyPAB18081 ALK polyclonal antibodyPAB18082 ALK polyclonal antibodyPAB25024 ALK polyclonal antibodyPAB25504 ALK polyclonal antibodyPAB26992 ALK polyclonal antibodyPAB29294 ALK (phospho Y1096) polyclonal antibodyPAB29642 ALK (phospho Y1604) polyclonal antibodyPAB30435 ALK polyclonal antibodyPAB8535 ALK polyclonal antibodyRAB00417 ALK recombinant monoclonal antibody, clone ALK1/2766RRAB00418 ALK recombinant monoclonal antibody, clone ALK1/2766RRAB00550 ALK recombinant monoclonal antibody, clone ALK/3218RTM0004 Protein BlueRed Marker