Description

TestedandCertifiedforinvivoimaging(See"LuciferinFAQ"inadditionalinformation)

Luciferinisacommonbioluminescentreporterusedforinvivoimagingoftheexpressionofluciferase.ThiswatersolublesubstrateforthefireflyluciferaseenzymeutilizesATPandMg²⁺ascofactorstoemitacharacteristicyellow-greenemissioninthepresenceofoxygen,whichshiftstoredlightinvivoat37°C.ThroughtheutilizationofATP,thereactioncanbefurtherusedtoindicatethepresenceofenergyorlifeinordertofunctionasalife-deathstain.

Luciferinisacommonreagentusedthroughoutthebiotechnologyfieldandspecificallyforinvivoimaging.Luciferaselabeledtumorcells,stemcellsorinfectiousdiseasesareofteninoculatedintoresearchanimalssuchasratsormiceforinvestigation.Theinjectionofluciferinallowsforthereal-time,noninvasivemonitoringofdiseaseprogressionand/ordrugefficacyinthesemodelsystemsthroughBioluminescenceImaging(BLI).

Luciferinisalsocommonlyusedforinvitroresearch,includingluciferaseandATPassays,genereporterassays,highthroughputsequencingandvariouscontaminationassays.

FireflyLuciferinisidenticaltoBeetleLuciferin.(See"AdditionalInformation"forstructures.)

ProductSpecifications:
D-Luciferin,PotassiumSalt,
4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylicacidpotassiumsalt,

MOLECULARBIOLOGYGRADE(PremiumPure)

Formula:KC₁₁H₇N₂O₃S₂

MW:318.42g/mol

Color:Lightyellowpowder

Purity:>99%pure.(QualityverifiedbynineindependentcriteriaincludingHPLCandFTIR.)

Storage/Handling:Storedesiccatedat-20°C.Protectfromlight.

PubChemChemicalID:44134804