Mouse 20S Proteasome Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-355
Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.6, 150 mM NaCl, 1 mM DTT. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: 20S Proteasome
The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins. It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28 alpha/beta complex, or the PA28 gamma complex (1). The 20S Proteasome is composed of 28 subunits arranged into four stacked rings (2,3). The outer rings, containing seven subunits each, are composed of closely-related but non-identical alpha subunits. The amino-terminal tails of the alpha subunits form a gate that restricts substrate entry into the catalytic core. The inner rings, also containing seven subunits each, are composed of closely-related but non-identical beta subunits. The amino-terminal tails of six of the beta subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention (4). The 20S Proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 Activator alpha (5).
- Stadtmueller, B.M. & C.P. Hill (2011) Mol. Cell 41:8.
- Kim, H.M. et al. (2011) Biochim. Biophys. Acta 1809:67.
- Xie, Y. (2010) J. Mol. Cell Biol. 2:308.
- Kisselev, A.F. et al. (2012) Chem. Biol. 19:99.
- Ma, C.P. et al. (1992) J. Biol. Chem. 267:10515.
Citations for Mouse 20S Proteasome Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- Effect of Fish Oil vs. Resolvin D1, E1, Methyl Esters of Resolvins D1 or D2 on Diabetic Peripheral NeuropathyAuthors: A Obrosov, LJ Coppey, H Shevalye, MA YorekJ Neurol Neurophysiol, 2017;8(6):.Species: MouseSample Types: Tissue HomogenatesApplications: Bioassay
- Denervation-Induced Activation of the Standard Proteasome and ImmunoproteasomePLoS ONE, 2016;11(11):e0166831.Species: MouseSample Types: Whole TissueApplications: Bioassay
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根据PTK是否存在于细胞膜受体可将其分成受体型和非受体型。向左转|向右转
protease
水解蛋白质肽键的一类酶的总称。按其水解多肽的方式,可以将其分为内肽酶和外肽酶两类。内肽酶将蛋白质分子内部切断,形成分子量较小的月示和胨。外肽酶从蛋白质分子的游离氨基或羧基的末端逐个将肽键水解,而游离出氨基酸,前者为氨基肽酶后者为羧基肽酶。按其活性中心和最适pH值,又可将蛋白酶分为丝氨酸蛋白酶、巯基蛋白酶、金属蛋白酶和酸性蛋白酶。按其反应的最适pH值,分为酸性蛋白酶、中性蛋白酶和碱性蛋白酶。工业生产上应用的蛋白酶,主要是内肽酶。
蛋白激酶
protein kinase
又称蛋白质磷酸化酶(protein phosphakinase)。一类催化蛋白质磷酸化反应的酶。
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