S. aureus Topoisomerase IV
Click on the product headings below to view details and order online.
S. aureus Topoisomerase IV
Topo IV (from Staphylococcus aureus) is prepared by overexpressing the subunits in E. coliand purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
See technical documents below for more detailed information and lot specific activities.
Technical Documents
S. aureus Topoisomerase IV Relaxation Assay Kits
These contain S. aureus topo IV and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.
Technical Documents
S. aureus Topoisomerase IV Decatenation Assay Kits
These contain S. aureus topo IV and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
Technical Documents
S. aureus Topoisomerase IV Cleavage Assay Kits
These kits are designed specifically for cleavage reactions. They contain S. aureus topo IV enzyme, supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to linearised pBR322 marker.
Cleavage specific enzyme available separately on request.
Technical Documents
S. aureus Topoisomerase IV Assay Kit for Cell Extracts
These kits are designed for assaying cell extracts and partially purified fractions containing over-expressed S. aureus topo IV and contain supercoiled DNA substrate, Assay buffer, Dilution buffer, control relaxed DNA and stop buffer/loading dye.
Technical Documents
S. aureus Topoisomerase IV ATPase kit
These kits can be used to test the effects of potential ATPase inhibitors. For example, the coumarin drugs such as novobiocin inhibit the action of topoisomerase IV by competitively inhibiting the hydrolysis of ATP thus preventing supercoiling.
These assays are microtitre plate-based and thus large numbers of compounds can be screened in a relatively short period of time. They also continuous assays which can provide more information than an end point assay.
Technical Documents
High / Medium-Throughput Assay Kit - S. aureus Topoisomerase IV
The kit is supplied with sufficient S. aureus topo IV enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.
More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".
Kit issued with limited licence for individual use only.
Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)
Technical Documents
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
根据PTK是否存在于细胞膜受体可将其分成受体型和非受体型。向左转|向右转
protease
水解蛋白质肽键的一类酶的总称。按其水解多肽的方式,可以将其分为内肽酶和外肽酶两类。内肽酶将蛋白质分子内部切断,形成分子量较小的月示和胨。外肽酶从蛋白质分子的游离氨基或羧基的末端逐个将肽键水解,而游离出氨基酸,前者为氨基肽酶后者为羧基肽酶。按其活性中心和最适pH值,又可将蛋白酶分为丝氨酸蛋白酶、巯基蛋白酶、金属蛋白酶和酸性蛋白酶。按其反应的最适pH值,分为酸性蛋白酶、中性蛋白酶和碱性蛋白酶。工业生产上应用的蛋白酶,主要是内肽酶。
蛋白激酶
protein kinase
又称蛋白质磷酸化酶(protein phosphakinase)。一类催化蛋白质磷酸化反应的酶。
暂无品牌问答