首页 > Millipore > Millipore/04-1074 | Anti-PECAM-1 [CD31] Antibody, clone EP3095, rabbit monoclonal/04-1074/100 µL

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Millipore/04-1074 | Anti-PECAM-1 [CD31] Antibody, clone EP3095, rabbit monoclonal/04-1074/100 µL

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DescriptionCatalogueNumber04-1074DescriptionAnti-PECAM-1[CD31]Antibody,cloneEP3095,rabbitmonoclonalAlternateNamesCD31antigenPECAM-1CD31/EndoCAM,adhesionmoleculeBackgroundInformationCD31,alsoknownasplateletendothelialcelladhesionmolecule1(PECAM1)isatypeIintegralmembraneglycoprotein,andamemberoftheimmunoglobulinsuperfamilyofcellsurfacereceptors.PECAM-1isconstitutivelyexpressedonthesurfaceofendothelialcellswhereitisconcentratedatcelljunctions.PECAM-1isalsoexpressedatlowlevelsonallleukocytesandplatelets.HomotypicinteractionofCD31isimportantincell-cellinteraction,cell-matrixinteractionandsignaltransduction.ProductInformationFormatUnpurifiedControlTHP-1andJurkatcelllysatesPresentationRabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA.StorageandShippingInformationStorageConditionsStablefor1yearat-20ºCfromdateofreceipt.HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.ApplicationsApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.Anti-PECAM-1[CD31]Antibody,cloneEP3095,RabbitdetectslevelofPECAM-1[CD31]&hasbeenpublished&validatedforuseinWB,IH(P),FC.KeyApplicationsWesternBlottingImmunohistochemistry(Paraffin)FlowCytometryApplicationNotesImmunohistochemistryAnalysis:1:250-500dilutionfromapreviouslotdetectedPECAM1inhumanuterusandtonsiltissue.FlowCytometry:1:70dilutionfromapreviouslotwasusedonFC.BIOLOGicalInformationImmunogenSyntheticpeptidecorrespondingtoresiduesonhumanPECAM-1.CloneEP3095HostRabbitSpecificityThisantibodyrecognizesPECAM1.IsotypeIgGSpeciesReactivityHumanAntibodyTypeMonoclonalAntibodyEntrezGeneNumberNP_000433.3GeneSymbolCD31CD31/EndoCAMEndoCAMGPIIAPECAM-1PurificationMethodUnpurifiedUniProtNumberP16284UniProtSummaryFUNCTION:Thisproteinisacelladhesionmoleculeexpressedonplateletsandatendothelialcellintercellularjunctions.SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.TISSUESPECIFICITY:Longisoformpredominatesalltissuesexamined,isoformDelta12wasdetectedonlyintracheaandisoformDelta14-15onlyinlung,isoformDelta14wasdetectedinalltissuesexaminedwiththestrongestexpressioninheart.PTM:PhosphorylatedonSerandTyrresiduesaftercellularactivation.SEQUENCESIMILARITIES:Contains6Ig-likeC2-type(immunoglobulin-like)domains.MolecularWeight130kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation

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