DescriptionCatalogueNumberAB1530BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-PeripherinAntibodyBackgroundInformationPeripherinisa56-58kDatypeIIIintermediatefilamentprotein(Portieretal.1983),expressedinmotor,sensoryandsympatheticsystemneuronsaswellasneuroendocrineskincarcinomasandcertainmelanocytictumors.Itisoftenusedanindicatorofentericganglioncelldensityinnormalanddiseased(e.g.Hirschsprung"sdisease)states.AbnormalinclusionscontainingperipherinhavealsobeenreportedinALS.Duringnormal,lateneurogenesisperipherinappearsaftertheexpressionofnestin,vimentin,alpha-internexinandneurofilamentsandisinvolvedinthedifferentiationofneurons,namelyoftheperipheralnervoussystem,andalsoprobablyinaxonalregeneration.Peripherinisnotrelatedtoperipherin-RDS,aphotoreceptorproteinassociatedwithretinaldegenerationandblindness.ProductInformationFormatSerumControlRatsensoryneurons,ratspinalcordhomogenateandperipheralnervehomogenate.PresentationRabbitpolyclonalantisera,nopreservatives.StorageandShippingInformationStorageConditionsStablefor1yearat-20°Cinundilutedaliquotsfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.ApplicationsApplicationThisAnti-PeripherinAntibodyisvalidatedforuseinIH,IH(P),WBforthedetectionofPeripherin.KeyApplicationsImmunohistochemistryImmunohistochemistry(Paraffin)WesternBlottingApplicationNotesAdditionalResearchApplicationsImmunohistochemistry:Apreviouslotofthisantibodywasusedat1:100-1:200dilution.Itissuggestedthattheantibodybeusedonfrozensectionsfixedinacetoneat-20°C.AB1530willworkontissuefixedforonehourorlessinfresh4%paraformaldehyde.Ithasbeenreportedthatthisantibodycanbeusedonparaffinembeddedtissuesections.SeeCell&TissueResearch(1997)288:11-23&EuropeanJ.Dermatology(1998)8:339-342.ElectronMicroscopy:ApreviouslotofthisantibodywasusedonElectronMicroscopy.Optimalworkingdilutionsandprotocolsmustbedeterminedbyenduser.BIOLOGicalInformationImmunogenElectrophoreticallypuretrp-E-peripherinfusionprotein[Dev.BrainRes.(1990)57:235-248],containingallbutthe4Nterminalaminoacidsofratperipherin.FusionproteinpurifiedfrombacterialinclusionbodiesbyDEAE-cellulosechromatographyin6MureafollowedbypreparativeSDS-PAGE.ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostRabbitSpecificityRecognizesPeripherin.Theantibodystainsa~57kDabandcleanlyandspecificallyanddoesnotstainvimentin,GFAP,alpha-internexinoranyoftheneurofilamentsubunits.Strongstainingonrat,mouse,human,pigandcowperipherin.Doesnotstainchicken,quailorothermoredistantlyrelatedspecieswhichappeartolackperipherin.SpeciesReactivityHumanRatMouseBovinePigSpeciesReactivityNoteStrongstainingonratandhuman.Cross-reactswithmouse,pig,andbovine.Otherspeciesnottested.AntibodyTypePolyclonalAntibodyEntrezGeneNumberNP_006253EntrezGeneSummaryThisgeneencodesacytoskeletalproteinfoundinneuronsoftheperipheralnervoussystem.TheencodedproteinisatypeIIIintermediatefilamentproteinwithhomologytoothercytoskeletalproteinssuchasdesmin,andisadifferentproteinthattheperipherinfoundinphotoreceptors.Mutationsinthisgenehavebeenassociatedwithsusceptibilitytoamyotrophiclateralsclerosis.GeneSymbolPRPHPeripherinPRPH1peripherinNEF4PurificationMethodUnpurifiedUniProtNumberP41219UniProtSummaryFUNCTION:SwissProt:P41219#Class-IIIneuronalintermediatefilamentprotein.SIZE:470aminoacids;53651DaSIMILARITY:SwissProt:P41219##Belongstotheintermediatefilamentfamily.MolecularWeight56-58kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation