DescriptionCatalogueNumber04-218BrandFamilyUpstateTradeNameUpstateDescriptionAnti-CREBAntibody,cloneE306,rabbitmonoclonalAlternateNamescAMPresponsiveelementbindingproteinactivetranscriptionfactorCREBtransactivatorproteinBackgroundInformationCREB,cyclicAMP(cAMP)responseelement-bindingprotein,isaubiquitouslyexpressedbasicleucinezippertranscriptionfactorthatfunctionsinglucosehomeostasisandstimulatestheexpressionofnumerousgenesinresponsetogrowthfactors,hormones,neurotransmitters,ionfluxes,stresssignals,andhasbeenimplicatedinmemorybecauseifitsapparentroleinsynapticplasticity.CREBfunctionseitherbyitselforwithrelatedfamilymembers,whichincludeCREB,CREM,andATF-1,byforminghomo-andheterodimersthroughtheirbasicleucinezipper(bZIP)domains.TheCREBsignalingpathwayisactivatedbyextracellularligandsthatbindtocellsurfacereceptorsandrelaytheirmessagethroughvariousintracellularsecondmessengersresultingultimatelyintheactivationofnuclearCREBthroughphosphorylationofthetransactivationdomainatserine1332,4.TheclassicalmodelofCREBactivationisthroughPKA(cAMP-dependentproteinkinase).Inthenon-activestate,PKAresidesinthecytoplasmasaninactiveheterotetramerofpairedregulatoryandcatalyticsubunits.StimulationofcAMPcausesthereleasesofthecatalyticsubunitsofPKAthatpassivelydiffuseintothenucleusandinitiatesgeneexpressionbyphosphorylatingCREBatitstransactivationdomainonserine1331,2,3,4.CREBmediatestheactivationofcAMP-responsivegenesbybindingasadimertoaconservedcAMP-responsiveelement(CRE),5’-TGACGTCA-3’,locatedwithinthepromoterregionoftargetgenes.Targetgeneactivationisterminatedbytheserine/threoninephosphatasesPP-1-mediateddephospho-rylationofCREB.ProductInformationFormatCultureSupernatantControlA431wholecelllysate.Presentation100μLofrabbitmonoclonalIgGin60%storagebuffer(50mMTris-Glycine(pH7.4),0.15MNaCl,0.01%sodiumazideand0.05%BSA)and40%glycerol.StorageandShippingInformationStorageConditionsStablefor1yearat-20ºCfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.ApplicationsApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.Anti-CREBAntibody,cloneE306,rabbitdetectslevelofCREB&hasbeenpublished&validatedforuseinFC,IC,IF,IH(P),IP&WB.KeyApplicationsFlowCytometryImmunocytochemistryImmunofluorescenceImmunohistochemistry(Paraffin)ImmunoprecipitationWesternBlottingApplicationNotesFlowCytometry:1:40dilutionfromapreviouslotwasusedonflowcytometry.Immunohistochemistry:1:250-500dilutionfromapreviouslotwasusedonimmunohistochemistry.Immunoprecipitation:1:80dilutionfromapreviouslotwasusedonimmunoprecipitation.Immunocytochemistry:1:250-500dilutionfromapreviouslotwasusedonimmunocytochemistry.BIOLOGicalInformationImmunogenKLHconjugatedtoCREBlinearpeptide.EpitopeInternalCloneE306HostRabbitSpecificityThisantibodyrecognizesCREB.IsotypeIgGSpeciesReactivityHumanMouseRatSpeciesReactivityNoteProventoreactwithhuman,ratandmouse.AntibodyTypeMonoclonalAntibodyEntrezGeneNumberNM_004379GeneSymbolMGC9284CREBUniProtNumberP16220UniProtSummaryFunction:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity.Subunitstructure:InteractswithPPRC1.BindsDNAasadimer.Thisdimerisstabilizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity.Subcellularlocation:Nucleus.Post-translationalmodification:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR.Sequencesimilarities:BelongstothebZIPfamily.Contains1bZIPdomain.Contains1KID(kinase-inducIBLe)domain.MolecularWeight~43kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation