DescriptionCatalogueNumberAB1554Replaces04-1111BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-NerveGrowthFactorReceptorAntibody,p75AlternateNamesCD271antigenLowaffinityneurotrophinreceptorp75NTRLow-affinitynervegrowthfactorreceptorNGFreceptorlowaffinitynervegrowthfactorreceptornervegrowthfactorreceptornervegrowthfactorreceptor(TNFRsuperfamily,member16)p75ICDBackgroundInformationNerveGrowthFactorReceptor,alsotermedNGFRp75,p75NTR,orCD271isthelowaffinityNGFR(LNGFR)whichbindsNGFandotherneurotrophinsincludingBDNF,NT3,NT4/5withsimilar,lowaffinity.NGFRp75isa75kDatransmembraneglycoprotein(399a.a.)consistingofanextracellulardomain(222a.a.)whichcontainsfourcysteinerichdomainsresponsIBLeforligandbinding,atransmembranedomain(22a.a.),andacytoplasmicdomain(155a.a.).NGFRp75ismainlyexpressedinSchwanncellsandneuronsandinavarietyofnonneuronalcells.NGFRp75isnecessaryforregulatingneuronalgrowth,migration,differentiationandcelldeathduringdevelopmentofthecentralandperipheralnervoussystem.NGFRp75playsacentralroleintheregulationofcellnumberbyapoptosisinthedevelopingCNS.ProductInformationFormatSerumControlRatPC12cells.PresentationRabbitpolyclonalserumcontaining0.05%NaN3.StorageandShippingInformationStorageConditionsStablefor1yearat-20ºCfromdateofreceipt.ApplicationsApplicationAnti-NerveGrowthFactorReceptorAntibody,p75isanantibodyagainstNerveGrowthFactorReceptorforuseinIC,IH(P),andIP.KeyApplicationsImmunocytochemistryImmunohistochemistry(Paraffin)ImmunoprecipitationApplicationNotesImmunoprecipitation:1:500-1:5000dilutionofthisantibodyimmunoprecipitatedNGFReceptorp75from10µgofPC12celllysate.Immunohistochemistry(paraffin):1:50and1:300dilutionfromapreviouslotdetectedNGFReceptorp75usingIHC-SelectDetectionwithHRP-DAB.Immunocytochemistry:1:500dilutionfromapreviouslotdetectedNGFReceptorp75inratPC12cells.BIOLOGicalInformationImmunogenRecombinantmouseNGFReceptorp75extracellularfragment.EpitopeextracellularConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostRabbitSpecificityp75nervegrowthfactorreceptor(p75Neurotrophinreceptor,lowaffinityneurotrophinreceptor),extracellulardomain.SpeciesReactivityHumanMouseRatSpeciesReactivityNoteIthasbeenreportedthatthisantibodydoesnotrecognizeratp75byWesternblot.SeeMAB365forratreactiveantibodyusingWesternblotting.AntibodyTypePolyclonalAntibodyEntrezGeneNumberNP_150086.2EntrezGeneSummaryNervegrowthfactorreceptorcontainsanextracellulardomaincontainingfour40-aminoacidrepeatswith6cysteineresiduesatconservedpositionsfollowedbyaserine/threonine-richregion,asingletransmembranedomain,anda155-aminoacidcytoplasmicdomain.Thecysteine-richregioncontainsthenervegrowthfactorbindingdomain.GeneSymbolNGFRp75(NTR)TNFRSF16Gp80-LNGFRCD271PurificationMethodUnpurifiedUniProtNumberQ9Z0W1UniProtSummaryFUNCTION:SwissProt:P08138#LowaffinityreceptorwhichcanbindtoNGF,BDNF,NT-3,andNT-4.Canmediatecellsurvivalaswellascelldeathofneuralcells.SIZE:427aminoacids;45183DaSUBUNIT:Homodimer;disulfide-linked.Interactswithp75NTR-associatedcelldeathexecutor.InteractswithTRAF2,TRAF4,TRAF6,PTPN13andRANBP9.InteractsthroughTRAF6withSQSTM1whichbridgesNGFRtoNTRK1.InteractswithBEX1andNGFRAP1/BEX3.SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.DOMAIN:SwissProt:P08138DeathdomainisresponsibleforinteractionwithRANBP9.PTM:N-andO-glycosylated.&O-linkedglycansconsistofGal(1-3)GalNAccoreelongatedby1or2NeuNAc.&Phosphorylatedonserineresidues.SIMILARITY:Contains1deathdomain.&Contains4TNFR-Cysrepeats.MolecularWeight44.7kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation