DescriptionCatalogueNumberMAB1398ZReplaces04-1074BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-PECAM-1Antibody,clone2H8,AzideFreeAlternateNamesCD31PECAM-1PlateletendothelialcelladhesionmoleculeBackgroundInformationCD31,alsoknownasplateletendothelialcelladhesionmolecule1(PECAM1),isatypeIintegralmembraneglycoproteinandamemberoftheimmunoglobulinsuperfamilyofcellsurfacereceptors.Itisconstitutivelyexpressedonthesurfaceofendothelialcells,andconcentratedatthejunctionbetweenthem.Itisalsoweaklyexpressedonmanyperipherallymphoidcellsandplatelets.CD31hasbeenusedtomeasureangiogenesisinassociationwithtumorrecurrence.OtherstudieshavealsoindicatedthatCD31andCD34canbeusedasMarkersformyeloidProgenitorcellsandrecognizedifferentsubsetsofmyeloidleukemiainfiltrates(granularsarcomas).ProductInformationFormatPurifiedControlMousecelllungtissuePresentationProteinAPurifiedmouseimmunoglobulinin20mMsodiumphosphate,250mMNaCl,pH.7.6,containingnopreservatives.StorageandShippingInformationStorageConditionsMaintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ApplicationsApplicationAnti-PECAM-1Antibody,clone2H8,AzideFreedetectslevelofPECAM-1&hasbeenpublished&validatedforuseinElecronMicroscopy,FlowCytometry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,andNeutralizationAssay.KeyApplicationsElectronMicroscopyFlowCytometryImmunocytochemistryImmunofluorescenceImmunohistochemistryImmunoprecipitationNeutralizationAssayApplicationNotesImmunofluorescenceAnalysis:Representativelotsimmunostainedendothelialbloodvesselcellsin4%paraformaldehyde-fixedfrozentumorsectionsfrommiceanddogsasameansforassessinglymphvesseldensityintumorxenograftsbyfluorescentimmunohistochemistry(Donat,U.,etal.(2012).PLoSOne.7(9):e45942;Gentschev,I.,etal.(2012).PLoSOne.7(5):e37239).ImmunofluorescenceAnalysis:Arepresentativelotimmunostainedendothelialcellsinformalin-fixed,paraffin-embeddedmurineperitoneumsectionsbyfluorescentimmunohistochemistry(Tanabe,K.,etal.(2007).KidneyInt.71(3):227-238).ImmunocytochemistryAnalysis:Arepresentativelotimmunostained4%paraformaldehyde-fixedendothelialcellsdifferentiatedfrommurineESCsincultures(Purpura,K.A.,etal.(2008).StemCells.26(11):2832-2842).ImmunocytochemistryAnalysis:Clone2H8immunostained3%paraformaldehyde-fixed,0.5%NP-40-permeABIlizedCOS-7transfectantsexpressingmurine,butnothuman,CD31byfluorescentimmunocytochemistry(Bogen,S.A.,etal.(1992).Am.J.Pathol.141(4):843-854).NeutralizationAnalysis:Arepresentativelot,wheninjectedintravenouslyinmice,blockedleukocyteemigrationinresponsetothioglycollateinjection(Bogen,S.,etal.(1994).J.Exp.Med.179(3):1059-1064).NeutralizationAnalysis:ArepresentativelotblockedmurinePECAM-mediatedaggregationofmouseLcells(Xie,Y.,andMuller,W.A.(1993).Proc.Natl.Acad.Sci.U.S.A.190(12):5569-5573).FlowCytometryAnalysis:ArepresentativelotdetectedtheexpressionofexogenouslytransfectedmurineCD31onthesurfaceofmouseLcells(Xie,Y.,andMuller,W.A.(1993).Proc.Natl.Acad.Sci.U.S.A.190(12):5569-5573).FlowCytometryAnalysis:Clone2H8detectedCD31immunoreactivityonthesurfaceofvariousmurineB-andT-celllines(Bogen,S.A.,etal.(1992).Am.J.Pathol.141(4):843-854).ImmunoprecipitationAnalysis:Arepresentativelotimmunoprecipitatedexogenouslyexpressedmurine,butnothuman,CD31fromCOS-7transfectants(Xie,Y.,andMuller,W.A.(1993).Proc.Natl.Acad.Sci.U.S.A.190(12):5569-5573).ImmunoprecipitationAnalysis:Clone2H8immunoprecipitatedCD31frommurineCTLLTlymphocytesandSp2/0Blymphocytes(Bogen,S.A.,etal.(1992).Am.J.Pathol.141(4):843-854).ElectronMicroscopyAnalysis:Clone2H8wasbiotinylatedandimmunostainedlymphocytesin0.01%glutaraldehyde-/2%formaldehyde-fxedfrozenreactivelymphnodetissuesfromKLH-immunizedmice(Bogen,S.A.,etal.(1992).Am.J.Pathol.141(4):843-854).ImmunohistochemistryAnalysis:Clone2H8immunostainedlymphocytesadherenttothesinusoidalandpostcapillaryvenularendotheliumoffrozenreactivelymphnodetissuesfromKLH-immunizedmice(Bogen,S.A.,etal.(1992).Am.J.Pathol.141(4):843-854).BIOLOGicalInformationImmunogenD10.G4.1murineTcells(Bogen,S.A.,etal.(1992).Am.J.Pathol.141(4):843-854).EpitopeExtracellulardomain.Clone2H8ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostArmenianHamsterSpecificityReactswiththe130-140kDaprotein,PECAM-1foundonmousemonocytes,platelets,granulocytesandendothelialcells.2H8effectivelyinhibitstransmigrationofactivatedpolymophonuclearcellsandmonocytesacrosstheendothelium.Inamousemodelforacuteperitonis-2H8blocksacuteinflammation.IsotypeIgGSpeciesReactivityMouseCanineAntibodyTypeMonoclonalAntibodyEntrezGeneNumberNM_001305157GeneSymbolPecamPecam-1Pecam1PurificationMethodProteinAPurfiedUniProtNumberQ08481PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation