DescriptionCatalogueNumberECM460BrandFamilyChemicon®TradeNameChemiconDescriptionαVβ3/PECAMInvestigatorKitOverviewChemiconInvestigatorKitsforIntegrinResearchareacost-effectivesourceofreagentsforallinvestigatorsinvolvedwiththisrapidlyexpandingfieldofstudy.ThealphaVbeta3/PECAMIntegrinInvestigatorKitisdesignedforresearchfocusedonthepresenceofthehumanalphaVbeta3integrinsubunitonPECAM-1[CD31]expressingcells.ThealphaVbeta3/PECAMIntegrinInvestigatorKitcontains100mgeachofCy5-conjugatedmouseanti-integrinalphaVbeta3andFluorescein-conjugatedmouseanti-PECAM-1[CD31]monoclonalantibodies.Integrinsbelongtoalargefamilyofcellsurfaceproteinscommonlyreferredtoasglycoproteins.Integrinsarecomposedofnoncovalentlyassociatedalphaandbetasubunitswhichmakeupheterodimers:thelargertermedalphachainandthesmallerbetachain.Atleast16alphasubunitsand8betasubunitsareexpressed,whichcangiveriseto22differentintegrins.Integrinsarethoughttoplaycriticalrolesincellmigration,differentiation,andsurvival.Thereisnowconsiderableevidencethatthesetransmembranereceptorsplayanimportantroleintheregulationofgeneexpressionandsignaltransduction.Excellentreviewsofintegrinstructure,function,andligandspecificitymaybefoundin:Hynes,R.1992.Cell69:11-25;Kramer,R.1993."IntegrinStructureandLigandSpecificityinCell-MatrixInteractions"inMolecularandCellularAspectsofBasementMembranes.AcademicPress.Thereagentsinthiskitarenotapplicationspecificbutrathermaybeusedinavarietyofprocedures,dependingupontheneedsoftheindividualinvestigator.ThecombinationofCy5andFluoresceinlabelspermitssimultaneouslabelingofIntegrinalphaVbeta3andPECAMinflowcytometryorimmunofluorescence.Thiskitandreagentsisnotintendedforuseinadiagnosticorclinicalsetting.ProductInformationComponentsIntegrinalphaVbeta3Cy5-conjugatedMouseMonoclonalAntibody(PartNo.MAB1976S-100)-1tube,100μg.PECAM[CD31]Fluorescein-conjugatedMouseMonoclonalAntibody(PartNo.MAB2148F-100)-1tube,100μg.Presentation100μgeachofmonoclonalstoalphaVbeta3andPECAM,labeledwithCY5andFITC,respectively.StorageandShippingInformationStorageConditionsStoreantibodiesat2-8°C.Protectfromlight.ImportantNote:Duetothesmallvolumescontainedwithinthekit,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidentrappedinthecontainer"scapduringshipment.ApplicationsKeyApplicationsELISAImmunocytochemistryImmunohistochemistryImmunoprecipitationFlowCytometryAffectsFunctionBIOLOGicalInformationSpeciesReactivityHumanEntrezGeneNumberNM_002210.2EntrezGeneSummaryITAGVencodesintegrinalphachainV.Integrinsareheterodimericintegralmembraneproteinscomposedofanalphachainandabetachain.TheI-domaincontainingintegrinalphaVundergoespost-translationalcleavagetoyielddisulfide-linkedheavyandlightchains,thatcombinewithmultipleintegrinbetachainstoformdifferentintegrins.Amongtheknownassociatingbetachains(betachains1,3,5,6,and8;"ITGB1","ITGB3","ITGB5","ITGB6",and"ITGB8"),eachcaninteractwithextracellularmatrixligands;thealphaVbeta3integrin,perhapsthemoststudiedofthese,isreferredtoastheVitronectinreceptor(VNR).Inadditiontoadhesion,manyintegrinsareknowntofacilitatesignaltransduction.GeneSymbolITGAVCD51DKFZp686A08142VNRAMSK8UniProtNumberP06756UniProtSummaryFUNCTION:SwissProt:P06756#Thealpha-Vintegrinsarereceptorsforvitronectin,cytotactin,fibronectin,fibrinogen,laminin,matrixmetalloproteinase-2,osteopontin,osteomodulin,prothrombin,thrombospondinandvonWillebrandfactor.TheyrecognizethesequenceR-G-Dinawidearrayofligands.IncaseofHIV-1infection,theinteractionwithextracellularviralTatproteinseemstoenhanceangiogenesisinKaposi"ssarcomalesions.SIZE:1048aminoacids;116038DaSUBUNIT:Heterodimerofanalphaandabetasubunit.Thealphasubunitiscomposedofanheavyandalightchainlinkedbyadisulfidebond.Alpha-Vassociateswitheitherbeta-1,beta-3,beta-5,beta-6orbeta-8subunit.InteractswithHIV-1Tat.SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.SIMILARITY:SwissProt:P06756##Belongstotheintegrinalphachainfamily.&Contains7FG-GAPrepeats.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation