首页 > Millipore > Millipore/CBL150F | Anti-CD3 Antibody, clone UCHT1, FITC conjugated/CBL150F/100 assays

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Millipore/CBL150F | Anti-CD3 Antibody, clone UCHT1, FITC conjugated/CBL150F/100 assays

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CBL150F

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DescriptionCatalogueNumberCBL150FReplacesCBL126FBrandFamilyChemicon®TradeNameChemiconDescriptionAnti-CD3Antibody,cloneUCHT1,FITCconjugatedAlternateNamesTCRProductInformationFormatFITCPresentationTheconjugateissuppliedasa100test/1mLvialinphosphatebufferedsalinecontaining10mMsodiumazideand1mg/mlbovineserumalbumin.Forflowcytometrywerecommendusing10μLofconjugatepertest.StorageandShippingInformationStorageConditionsStoreat+4°Cprotectedfromlight.DONOTFREEZE.Forlongtermuseandstoragealiquotconjugateintosmallvolumesandstoreat+4°C.ApplicationsApplicationAnti-CD3Antibody,cloneUCHT1,FITCconjugatedisanantibodyagainstCD3foruseinFC,IH.KeyApplicationsFlowCytometryImmunohistochemistryApplicationNotesFlowCytometryIdentificationofCD3positiveTlymphocytesbyindirectimmunofluorescencestainingIdentificationofnormalandmalignantTlymphocytesinfrozentissuesectionsImmunoprecipitationoftheCD3antigenMitogenicstimulationofTlymphocyteswithorwithoutthepresenceofIL-2LeukaemiaandlymphomaphenotypingOptimaldilutionsmustbedeterminedbyenduser.BIOLOGicalInformationImmunogenPurifiedhumanTcellsCloneUCHT1HostMouseSpecificityThisantibodyrecognizes22/26/30kDamolecularweightlymphocytesurfacemoleculesassociatedwiththeTcellantigenreceptorcomplex.ItreactswiththemajorityofperipheralbloodTlymphocytes,amajorproportionofthymocytes,themajorityofTcellchroniclymphocyticleukaemiacells,Sézaryleukaemiasandapproximately70%ofacutelymphoblasticleukaemiasofTcellorigin.Antigendistribution:Tcells100%PeripheralBloodlymphocytes75+7%Thymocytes68+5%GranulocytesMonocytesBcells(E-)NKcells(CD16+)KNOWNSPECIESCROSSREACTIVITY:NegativewithrhesusmonkeyperipheralbloodleucocytesinflowcytometryFUSIONPARTNER:P3x63Ag8.653myelomacelllineIsotypeIgG1SpeciesReactivityHumanAntibodyTypeMonoclonalAntibodyEntrezGeneNumberNM_000733.2EntrezGeneSummaryTheproteinencodedbythisgeneistheCD3-epsilonpolypeptide,whichtogetherwithCD3-gamma,-deltaand-zeta,andtheT-cellreceptoralpha/betaandgamma/deltaheterodimers,formstheT-cellreceptor-CD3complex.Thiscomplexplaysanimportantroleincouplingantigenrecognitiontoseveralintracellularsignal-transductionpathways.Thegenesencodingtheepsilon,gammaanddeltapolypeptidesarelocatedinthesameclusteronchromosome11.TheepsilonpolypeptideplaysanessentialroleinT-celldevelopment.Defectsinthisgenecauseimmunodeficiency.ThisgenehasalsobeenlinkedtoasusceptibilitytotypeIdiabetesinwomen.GeneSymbolCD3ET3ETCRENon-ReactiveSpeciesMonkeyUniProtNumberP07766UniProtSummaryFUNCTION:SwissProt:P07766#TheCD3complexmediatessignaltransduction.SIZE:207aminoacids;23147DaSUBUNIT:TheTCR/CD3complexofT-lymphocytesconsistsofeitheraTCRalpha/betaorTCRgamma/deltaheterodimercoexpressedatthecellsurfacewiththeinvariantsubunitsofCD3labeledgamma,delta,epsilon,zeta,andeta.SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.SIMILARITY:SwissProt:P07766##Contains1Ig-like(immunoglobulin-like)domain.&Contains1ITAMdomain.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation

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