DescriptionCatalogueNumberABN78DescriptionAnti-NeuNAntibody(rabbit)AlternateNamesRNAbindingproteinfox-1homolog3Fox-1homologCFox-3Hexaribonucleotide-bindingprotein3NeuNNeuronalnucleiBackgroundInformationRNAbindingproteinfox-1homolog3(UniProtQ8BIF2;alsoknownasFox-1homologC,Fox-3,Hexaribonucleotide-bindingprotein3,NeuN,Neuronalnuclei)isencodedbytheRbfox3(alsoknownasD11Bwg0517e,Hrnbp3)gene(GeneID52897)inmurinespecies.TheRNA-bindingFox(Rbfox)familyofsplicingfactorsiscomprisedofthreemembers,Rbfox1(Fox-1orA2BP1),Rbfox2(Fox-2orRBM9),andRbfox3(Fox-3,HRNBP3orNeuN).Rbfoxproteinsregulatesplicingofmanyneuronaltranscripts(pre-mRNAs)bybindingthesequence(U)GCAUGinintronsflankingalternativeexons.A(U)GCAUGmotifthatliesdownstreamofthealternativeexongenerallypromotesRbfox-dependentexoninclusion,whereasanupstreammotifwillusuallyrepressexoninclusion.OriginallycharacterizedasaMarkerofpost-mitoticneuronsandnamedneuronalnuclei(NeuN),Rbfox3playsaroleinpromotingneuronaldifferentiationthroughalternativesplicingofNumbpre-mRNAduringbraindevelopment.Rbfox3-knockoutinmiceisreportedtoresultindefectivehippocampalgeneexpression,defectsinsynaptictransmissionandplasticityinthedentategyrus,aswellasincreasedseizuresusceptibilityanddecreasedanxiety-relatedbehaviors.Likewise,humanRBFOX3genemutationshavebeenlinkedtoneurodevelopmentaldelay,cognitiveimpairments,autisticfeatures,andepilepsy.ProductInformationFormatAffinityPurifiedControlMousebrainnuclearextractPresentationPurifiedrabbitpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.StorageandShippingInformationStorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.ApplicationsApplicationAnti-NeuN(rabbit)Antibody,Cat.No.ABN78,isahighlyspecificrabbitpolyclonalantibodythattargetsNeuNandhasbeentestedinImmunocytochemistry,Immunofluorescence,Immunohistochemistry,andWesternBlotting.KeyApplicationsImmunocytochemistryImmunohistochemistryImmunofluorescenceWesternBlottingApplicationNotesImmunocytochemistryAnalysis:A1:500dilutionfromarepresentativelotdetectedNeuNinculturedembryonicE18rathippocampusneurons.ImmunohistochemistryAnalysis:A1:500dilutionfromarepresentativelotdetectedNeuN-positiveneuronsinhuman(cerebellumandcerebralcortex)andmouse(hippocampus)braintissuesections.ImmunocytochemistryAnalysis:Arepresentativelotsimmunostained4%paraformaldehyde-fixedneuronsisolatedfromsnailbrain(Nikolić,L.,etal.(2013).J.Exp.Biol.216(Pt18):3531-3541).ImmunofluorescenceAnalysis:RepresentativelotsdetectedNeuN-positiveneuronsinfrozenbrainsectionsfrom4%paraformaldehyde-perfusedmice(Cherry,J.D.,etal.(2015).J.Neuroinflammation.12:203;Huang,B.,etal.(2015).Neuron.85(6):1212-1226;Ataka,K.,etal.(2013).PLoSOne.8(11):e81744).ImmunofluorescenceAnalysis:ArepresentativelotsdetectedNeuN-positiveneuronsinformalin-fixedandparaffin-embeddedamyotrophiclateralsclerosis(ALS)humanbraintissuesections(Fratta,P.,etal.(2015).Neurobiol.Aging.36(1):546.e1-7).ImmunohistochemistryAnalysis:ArepresentativelotdetectedNeuN-positiveneuronsinratbraintissuesections(Mendonça,M.C.,etal.(2013).Toxins.5(12):2572-2588).WesternBlottingAnalysis:RepresentativelotdetectedNeuNinmouseandratbraintissuelysates(Huang,B.,etal.(2015).Neuron.85(6):1212-1226;Mendonça,M.C.,etal.(2013).Toxins.5(12):2572-2588).ThisrabbitpolyclonalantibodyisalsoavaiableasAlexaFluor®488(ABN78A4),biotin(ABN78B),andCy3(ABN78C3)conjugatesforimmunocytochemistry,immunofluorescence,immunohistochemistryapplications.BIOLOGicalInformationImmunogenGST-taggedrecombinantmouseNeuNN-terrminalfragment.EpitopeN-terminusConcentrationPleaserefertolotspecificdatasheet.HostRabbitSpecificityThisrabbitpolyclonalantibodytargetsNeuNN-terminalregionthatendsatthestartofRNArecognitionmotif(RRM).Targetregionispresentinallhuman,mouse,andratsplicedisoformsreportedbyUniProt(A6NFN3,Q8BIF2,D4A2H6,A0A0G2K2J4).SpeciesReactivityHumanMouseRatSnailSpeciesReactivityNoteHuman,Mouse,Rat,Snail.AntibodyTypePolyclonalAntibodyEntrezGeneNumberNP_001034256GeneSymbolRbfox3D11Bwg0517eHrnbp3PurificationMethodAffinityPurfiedUniProtNumberQ8BIF2MolecularWeight~48/42kDaobserved.33.87/35.12kDa(humanisoform1/2),40.61/39.05/35.64/34.07/35.63kDa(mouseisoform1/2/3/4/5),40.59kDa(rat;UniProtD4A2H6),34.05kDa(rat;UniProtA0A0G2K2J4)calculated.Uncharacterizedbandsmaybeobservedinsomelysate(s).PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation