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Millipore/IHCR1001-6 | Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60/IHCR1001-6/6 mL
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DescriptionCatalogueNumberIHCR1001-6BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-Neuron-SpecificNuclearProteinAntibody,prediluted,cloneA60AlternateNamesNeuNBackgroundInformationNeuNantibody(NEUronalNuclei;cloneA60)specificallyrecognizestheDNA-binding,neuron-specificproteinNeuN,whichispresentinmostCNSandPNSneuronalcelltypesofallvertebratestested.NeuNproteindistributionsareapparentlyrestrictedtoneuronalnuclei,perikaryaandsomeproximalneuronalprocessesinbothfetalandadultbrainalthough,someneuronsfailtoberecognizedbyNeuNatallages:INLretinalcells,Cajal-Retziuscells,Purkinjecells,inferiorolivaryanddentatenucleusneurons,andsympatheticganglioncellsareexamples(Mullenetal.,1992;Wolfetal.,1996).ImmunohistochemicallydetectableNeuNproteinfirstappearsatdevelopmentaltimepointsthatcorrespondwiththewithdrawaloftheneuronfromthecellcycleand/orwiththeinitiationofterminaldifferentiationoftheneuron(Mullenetal.,1992).ImmunoreactivityappearsaroundE9.5inthemouseneuraltubeandisextensivethroughoutthedevelopingnervoussystembyE12.5.Strongnuclearstainingsuggestsanuclearregulatoryproteinfunction;however,noevidencecurrentlyexistsastowhethertheNeuNproteinantigenhasafunctioninthedistalcytoplasmorwhetheritismerelysynthesizedtherebeforebeingtransportedbackintothenucleus.Nodifferencebetweenproteinisolatedfrompurifiednucleiandwholebrainextractonimmunoblotshasbeenfound(Mullenetal.,1992).ProductInformationFormatPurifiedPresentationLiquiddilutedinPBS,pH7.2withstABIlizers,0.2%Tween20,and0.1%ProClin300aspreservative.StorageandShippingInformationStorageConditionsMaintainat2-8°C.Refertovialforexpirationdating.ApplicationsApplicationAnti-Neuron-SpecificNuclearProteinAntibody,prediluted,cloneA60detectslevelofNeuron-SpecificNuclearProtein&hasbeenpublished&validatedforuseinIH(P).KeyApplicationsImmunohistochemistry(Paraffin)ApplicationNotesAntibodyispredilutedandreadytouseforImmunohistochemistryofformalin-fixed,paraffin-embeddedtissues.Pretreatment:HeatInducedEpitopeRetrieval(HIER).RecommendCitrateBuffer,pH6.0(Cat.No.21545).NosignalwasdetectedwithoutEpitoperetrieval.Incubation:30minuteswithIHCSelect®DetectionKits.NeuN,hasbeenpredilutedforuseastheprimaryantibodywithChemicon"sIHCSelect®DetectionKitsandProtocols(CatalogNos.DAB050,DET-HP1000,APR050,andDET-APR1000),butothersupplier"sIHCdetectionsystemsmaybeused.Foroptimizedprotocoldetails,visitwww.chemicon.comandselecttheprotocolslinkunderCat.No.IHCR1001-6.BIOLOGicalInformationImmunogenPurifiedcellnucleifrommousebrain.CloneA60HostMouseSpecificityneuron-specificproteinNeuNSpeciesReactivityAvianChickenFerretHumanMousePigRatPrimateSalamanderVertebratesAntibodyTypeMonoclonalAntibodyPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation
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