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Millipore/04-001 | Anti-JAK2 Antibody, clone 8E10.2/04-001/100 µg
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DescriptionCatalogueNumber04-001TradeNameUpstateDescriptionAnti-JAK2Antibody,clone8E10.2AlternateNamesJanuskinase2TyrosinekinaseBackgroundInformationJAK2(JanusKinase2)belongstotheemergingfamilyofnon-receptorJanustyrosinekinases,whichregulateaspectrumofcellularfunctionsdownstreamofactivatedcytokinereceptorsinthelympho-hematopoieticsystem.Immunologicalstimuli,suchasinterferonsandcytokines,inducerecruitmentofStattranscriptionfactorstocytokinereceptor-associatedJAK2.JAK2thenphosphorylatesproximalStatfactors,whichsubsequentlydimerizesandtranslocatestothenucleusandbindtoCISelementsupstreamoftargetgenepromoterstoregulatetranscription.ThecanonicalJAK/Statpathwayisintegraltomaintaininganormalimmunesystembystimulatingproliferation,differentiation,survivalandhostresistancetopathogens.AlteringJAK/Statsignalingtoreducecytokineinducedpro-inflammatoryresponsesrepresentsanattractivetargetforanti-inflammatorytherapies.ProductInformationFormatPurifiedControlL6celllysatePresentationPurifiedmousemonoclonalIgG1κinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazideStorageandShippingInformationStorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.ApplicationsApplicationAnti-JAK2Antibody,clone8E10.2detectslevelofJAK2&hasbeenpublished&validatedforuseinWB,IH(P).KeyApplicationsWesternBlottingImmunohistochemistry(Paraffin)ApplicationNotesWesternBlotAnalysis:0.5-1μg/mLofthislotwasusedtodetectJak2inL6celllysates.Immunohistochemistry(Paraffin):Apreviouslotofthisantibodywasusedinimmunohistochemistry(paraffin)stainingwitheptioperetrieval:HumanCancerArray.BIOLOGicalInformationImmunogenKLH-conjugatedlinearpeptide.Clone8E10.2ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostMouseSpecificityThisantibodyrecognizesJak2.IsotypeIgG1κSpeciesReactivityHumanMouseRatAntibodyTypeMonoclonalAntibodyEntrezGeneNumberNP_004963EntrezGeneSummaryThisgeneproductisaproteintyrosinekinaseinvolvedinaspecificsubsetofcytokinereceptorsignalingpathways.Ithasbeenfoundtobeconstituitivelyassociatedwiththeprolactinreceptorandisrequiredforresponsestogammainterferon.Micethatdonotexpressanactiveproteinforthisgeneexhibitembryoniclethalityassociatedwiththeabsenceofdefinitiveerythropoiesis.[providedbyRefSeq]GeneSymbolJAK2JAK-2PurificationMethodProteinGPurifiedUniProtNumberO60674MolecularWeight130kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation

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