DescriptionCatalogueNumber06-255BrandFamilyUpstateTradeNameUpstateDescriptionAnti-JAK2AntibodyBackgroundInformationTheJanusfamilyoftyrosinekinases(JAK)includesJAK1,JAK2,JAK3andTyk2.Thisfamilyassociateswithavarietyofcytokinereceptorsandisactivatedbyligandbinding.Uponligandbindingtocytokinereceptors,JAKsphosphorylatethemselvesandtheirassociatedreceptors.ThisprovidesmultiplebindingsitesforsignalingproteinscontainingSH2orotherphospho-tyrosine-bindingdomains,includingSTATs,Shc,insulinreceptorsubstratesandFAK.ProductInformationFormatSerumControlHeLaandJurkatcelllysatesPresentationProteinAPurifiedimmunoglobulinin30%glycerol,0.07MTris-glycine,pH7.4,0.105MNaCl,0.035%sodiumazideasapreservative.StorageandShippingInformationStorageConditionsMaintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ApplicationsApplicationAnti-JAK2AntibodyisanantibodyagainstJAK2foruseinIP&WB.KeyApplicationsImmunoprecipitationWesternBlottingBIOLOGicalInformationImmunogenpeptidecorrespondingtoresidues758-776ofmurineJAK2ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostRabbitSpecificityRecognizesmurine,human,andratJAK2,Mr130kDa.Additionalbandsmaybedetectedinsomelysates.IsotypeIgGSpeciesReactivityHumanMouseRatAntibodyTypePolyclonalAntibodyEntrezGeneNumberNM_004972EntrezGeneSummaryThisgeneproductisaproteintyrosinekinaseinvolvedinaspecificsubsetofcytokinereceptorsignalingpathways.Ithasbeenfoundtobeconstituitivelyassociatedwiththeprolactinreceptorandisrequiredforresponsestogammainterferon.Micethatdonotexpressanactiveproteinforthisgeneexhibitembryoniclethalityassociatedwiththeabsenceofdefinitiveerythropoiesis.GeneSymbolJAK2OTTHUMP00000043260JAK-2PurificationMethodUnpurifiedUniProtNumberO60674UniProtSummaryFUNCTION:SwissProt:O60674#Tyrosinekinaseofthenon-receptortype,involvedininterleukin-3andprobablyinterleukin-23signaltransduction.SIZE:1132aminoacids;130674DaSUBUNIT:InteractswithSIRPA(Bysimilarity).InteractswithIL23R,SKB1andSTAM2.SUBCELLULARLOCATION:Intracytoplasmicmembrane;Peripheralmembraneprotein(Bysimilarity).Note=Whollyintracellular,possIBLymembraneassociated(Bysimilarity).TISSUESPECIFICITY:Expressedinblood,bonemarrowandlymphnode.DOMAIN:SwissProt:O60674Possessestwophosphotransferasedomains.Thesecondoneprobablycontainsthecatalyticdomain(Bysimilarity),whilethepresenceofslightdifferencessuggestadifferentrolefordomain1.DISEASE:SwissProt:O60674#ChromosomalaberrationsinvolvingJAK2arefoundinbothchronicandacuteformsofeosinophilic,lymphoblasticandmyeloidleukemia.Translocationt(8;9)(p22;p24)withPCM1linkstheproteinkinasedomainofJAK2tothemajorportionofPCM1.Translocationt(9;12)(p24;p13)withETV6.&DefectsinJAK2areacauseofsusceptibilitytoBudd-Chiarisyndrome[MIM:600880].Budd-Chiarisyndromeisaspectrumofdiseasestates,includinganatomicabnormalitiesandhypercoagulabledisorders,resultinginhepaticvenousoutflowocclusion.Clinicalmanifestationsobservedinthemajorityofpatientsincludehepatomegaly,rightupperquadrantpain,andaBDominalascites.&DefectsinJAK2areassociatedwithpolycythemiavera(PV)[MIM:263300].PV,themostcommonformofprimarypolycythemia,iscausedbysomaticmutationinasinglehematopoieticstemcellleADIngtoclonalhematopoiesis.PVisamyeloproliferativedisordercharacterizedpredominantlybyerythroidhyperplasia,butalsobymyeloidleukocytosis,thrombocytosis,andsplenomegaly.FamilialcasesofPVareveryrareandusuallymanifestinelderlypatients.&DefectsinJAK2genemaybeacauseofessentialthrombocythemia(ET)[MIM:187950].ETischaracterizedbyelevatedplateletlevelsduetosustainedproliferationofmegakaryocytes,andfrequentlyleadtothromboticandhaemorrhagiccomplications.&DefectsinJAK2areassociatedwithfamilialmyelofibrosis[MIM:254450].Myelofibrosiswithmyeloidmetaplasiaisamyeloproliferativediseasewithannualincidenceof0.5-1.5casesper100,000individualsandageatdiagnosisaround60(anincreasedprevalenceisnotedinAshkenaziJews).Clinicalmanifestationsdependonthetypeofbloodcellaffectedandmayincludeanemia,pallor,splenomegaly,hypermetabolicstate,petechiae,ecchymosis,bleeding,lymphadenopathy,hepatomegaly,portalhypertension.&DefectsinJAK2areacauseofacutemyelogenousleukemia(AML)[MIM:601626].AMLisamalignantdiseaseinwhichhematopoieticprecursorsarearrestedinanearlystageofdevelopment.SIMILARITY:Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.JAKsubfamily.&Contains1FERMdomain.&Contains1proteinkinasedomain.&Contains1SH2domain.MolecularWeight125kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation