DescriptionCatalogueNumberAB9610BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-Olig-2AntibodyAlternateNamesOligodendrocytetranscriptionfactor2Oligo2ClassBbasichelix-loop-helixprotein1bHLHb1ClassEbasichelix-loop-helixprotein19bHLHe19ProteinkinaseC-bindingprotein2ProteinkinaseC-bindingproteinRACK17BackgroundInformationOlig2(oligodendrocytetranscriptionfactor2)isrequiredforoligodendrocyteandmotorneuronspecificationinthespinalcord,aswellasforthedevelopmentofsomaticmotorneuronsinthehindbrain.ItcooperateswithOLIG1toestablishthepMNdomainoftheembryonicneuraltube.ItisanantagoNISTofV2interneuronandofNKX2-2-inducedV3interneurondevelopment.TherestrictedexpressionofOlig2inoligodendrocytesmakesAB9610anexcellentMarkerofoligodendrogliaandoligodendrogliomasindevelopmentandmaturesystems.ProductInformationFormatPurifiedControlMousebrainwholecelllysate,PC12wholecelllysate,brain(rat)tissuelysatePresentationPurifiedrabbitpolyclonal.Liquidin100mMGlycine,200mMTris,pH8.0.StorageandShippingInformationStorageConditionsStablefor1yearat2-8ºCfromdateofreceipt.ApplicationsApplicationAnti-Olig-2AntibodyisanantibodyagainstOligodendrocytetranscriptionfactor2foruseinIC,IH,IH(P),IPandWB.KeyApplicationsImmunocytochemistryImmunohistochemistryImmunohistochemistry(Paraffin)ImmunoprecipitationWesternBlottingApplicationNotesWesternBlotAnalysis:1:2,500-1:5,000dilutionofapreviouslotwasusedinwesternblotonhuman,ratandmousecelllines(humanoligodendroglioma,ratprimaryneurepithelial,mousetransfected)andtissues(brainandspinalcord).Immunoprecipitation:1:500-1:1,000withhumanoligodendrogliomaandmousetransfectedcellline.Optimalworkingdilutionsmustbedeterminedbytheenduser.Immunocytochemistry:1:500-1:1,000dilutionofapreviouslotwasusedinimmunocytochemistryonhuman,ratandmousecelllines(humanoligodendroglioma,ratprimaryneurepithelial,mousetransfected)andtissues(brainandspinalcord).OptimalworkingdilutionsmustbedeterminedbytheenduserBIOLOGicalInformationImmunogenRecombinantmouseOlig-2ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostRabbitSpecificityRecognizesthe~32kDaOlig-2proteinbyWesternblot.SpeciesReactivityHumanMouseRatSpeciesReactivityNoteHuman,ratandmouse.Otherspecieshavenotbeentested.AntibodyTypePolyclonalAntibodyEntrezGeneNumberNM_005806.2EntrezGeneSummaryThisgeneencodesabasichelix-loop-helixtranscriptionfactorwhichisexpressedinoligodendroglialtumorsofthebrain.TheproteinisanessentialregulatorofventralneuroectodermalProgenitorcellfate.Thegeneisinvolvedinachromosomaltranslocationt(14;21)(q11.2;q22)associatedwithT-cellacutelymphoblasticleukemia.Itschromosomallocationiswithinaregionofchromosome21whichhasbeensuggestedtoplayaroleinlearningdeficitsassociatedwithDownsyndrome.GeneSymbolOLIG2bHLHB1RACK17OLIGO2BHLHB1PRKCBP2Oligo2PurificationMethodAmmoniumSulfatePrecipitationUniProtNumberQ13516UniProtSummaryFUNCTION:SwissProt:Q13516#Requiredforoligodendrocyteandmotorneuronspecificationinthespinalcord,aswellasforthedevelopmentofsomaticmotorneuronsinthehindbrain.CooperateswithOLIG1toestablishthepMNdomainoftheembryonicneuraltube.AntagonistofV2interneuronandofNKX2-2-inducedV3interneurondevelopment(Bysimilarity).SIZE:323aminoacids;32385DaSUBUNIT:InteractswithNKX2-2(Bysimilarity).SUBCELLULARLOCATION:Nucleus(Bysimilarity).Cytoplasm(Bysimilarity).Note=TheNLScontainedinthebHLHdomaincouldbemaskedinthenativeformandtranslocationtothenucleuscouldbemediatedbyinteractioneitherwithclassEbHLHpartnerproteinorwithNKX2-2(Bysimilarity).TISSUESPECIFICITY:Expressedinthebrain,inoligodendrocytes.Stronglyexpressedinoligodendrogliomas,whileexpressionisweaktomoderateinastrocytomas.Expressioninglioblastomashighlyvariable.DOMAIN:SwissProt:Q13516ThebHLHisessentialforinteractionwithNKX2-2(Bysimilarity).DISEASE:SwissProt:Q13516#AchromosomalaberrationinvolvingOLIG2maybeacauseofaformofT-cellacutelymphoblasticleukemia(T-ALL).Translocationt(14;21)(q11.2;q22)withTCRA.SIMILARITY:Contains1basichelix-loop-helix(bHLH)domain....MolecularWeight~32kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation