DescriptionCatalogueNumberAB980BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-MyelinBasicProteinAntibodyAlternateNamesMyelinA1proteinMyelinmembraneencephalitogenicproteinBackgroundInformationTheclassicgroupofMyelinbasicprotein(MBP)isoforms(isoforms4to14)arewithPLPthemostabundantproteincomponentsofthemyelinmembraneintheCNS.TheyhavearoleinbothitsformationandstABIlization.Thesmallerisoformsmighthaveanimportantroleinremyelinationofdenudedaxonsinmultiplesclerosis.ThenonclassicgroupofMBPisoforms(isoforms1to3/GolliMBPs)maypreferentiallyhavearoleintheearlydevelopingbrainlongbeforemyelination,maybeascomponentsoftranscriptionalcomplexes,andmayalsobeinvolvedinsignalingpathwaysinTcellsandneuralcells.Differentialsplicingeventscombinedtooptionalposttranslationalmodificationsgiveawidespectrumofisomers,eachofthemhavingmaybeaspecializedfunction.ProductInformationFormatPurifiedControlBraintissue,brainlysatePresentationLiquidin0.05mol/LTris-HCl,pH7.6with15mmol/Lsodiumazide.StorageandShippingInformationStorageConditionsStablefor1yearat2-8ºCfromdateofreceipt.ApplicationsApplicationThisAnti-MyelinBasicProteinAntibodyisvalidatedforuseinIH,IH(P),WBforthedetectionofMyelinBasicProtein.KeyApplicationsImmunohistochemistryImmunohistochemistry(Paraffin)WesternBlottingApplicationNotesImmunohistochemistry:1:200dilutionofapreviouslotwasusedbyPAPorABConformalinfixed,paraffin-embeddedhumanspinalcord.Pretreatmentoftissuewithproteolyticenzymeisnotrequired.WesternBlot:Adilutionof1:3000wasusedinTTBS,O/N4°Conapreviouslot.Optimalworkingdilutionsmustbedeterminedbytheenduser.BIOLOGicalInformationImmunogenHumanmyelinbasicproteinfrombrainConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostRabbitSpecificitySuitableforlabelingofoligodendrocytesandmyelinofwhitematterinbrainandspinalcord(graymatterstainslessabundantly).Alsostainsperipheralnerves.SpeciesReactivityHumanMouseRatAntibodyTypePolyclonalAntibodyEntrezGeneNumberNM_001025094.1EntrezGeneSummaryTheproteinencodedbytheclassicMBPgeneisamajorconstituentofthemyelinsheathofoligodendrocytesandSchwanncellsinthenervoussystem.However,MBP-relatedtranscriptsarealsopresentinthebonemarrowandtheimmunesystem.ThesemRNAsarisefromthelongMBPgene(otherwisecalled"Golli-MBP")thatcontains3additionalexonslocatedupstreamoftheclassicMBPexons.AlternativesplicingfromtheGolliandtheMBPtranscriptionstartsitesgivesriseto2setsofMBP-relatedtranscriptsandgeneproducts.TheGollimRNAscontain3exonsuniquetoGolli-MBP,splicedin-frameto1ormoreMBPexons.TheyencodehybridproteinsthathaveN-terminalGolliaasequencelinkedtoMBPaasequence.ThesecondfamilyoftranscriptscontainonlyMBPexonsandproducethewellcharacterizedmyelinbasicproteins.ThiscomplexgenestructureisconservedamongspeciessuggestingthattheMBPtranscriptionunitisanintegralpartoftheGollitranscriptionunitandthatthisarrangementisimportantforthefunctionand/orregulationofthesegenes.GeneSymbolMBPMGC99675Golli-mbpPurificationMethodAmmoniumSulfatePrecipitationUniProtNumberP02686UniProtSummaryFUNCTION:SwissProt:P02686#TheclassicgroupofMBPisoforms(isoform4-isoform14)arewithPLPthemostabundantproteincomponentsofthemyelinmembraneintheCNS.Theyhavearoleinbothitsformationandstabilization.Thesmallerisoformsmighthaveanimportantroleinremyelinationofdenudedaxonsinmultiplesclerosis.Thenon-classicgroupofMBPisoforms(isoform1-isoform3/Golli-MBPs)maypreferentiallyhavearoleintheearlydevelopingbrainlongbeforemyelination,maybeascomponentsoftranscriptionalcomplexes,andmayalsobeinvolvedinsignalingpathwaysinT-cellsandneuralcells.Differentialsplicingeventscombinedtooptionalpost-translationalmodificationsgiveawidespectrumofisomers,eachofthemhavingmaybeaspecializedfunction.InducesT-cellproliferation.SIZE:304aminoacids;33117DaSUBUNIT:Homodimer;isoform3existsasahomodimer.SUBCELLULARLOCATION:Myelinmembrane;Peripheralmembraneprotein;Cytoplasmicside.Note=Cytoplasmicsideofmyelin.TISSUESPECIFICITY:MBPisoformsarefoundinboththecentralandtheperipheralnervoussystem,whereasGolli-MBPisoformsareexpressedinfetalthymus,spleenandspinalcord,aswellasincelllinesderivedfromtheimmunesystem.DEVELOPMENTALSTAGE:Expressionturnsonabruptlyinfetusof14to16weeks.Evensmallerisoformsseemtobeproducedduringembryogenesis,someofthesepersistingintheadult.ExpressionofisoformMBP2ismoreevidentat16weeksanditsrelativeproportiondeclinedthereafter.PTM:SeveralchargeisomersofMBP;C1(themostcationic,leastmodified,andmostabundantform),C2,C3,C4,C5,C6,C7,C8-AandC8-B(theleastcationicform);areproducedasaresultofoptionalPTM,suchasphosphorylation,deamidationofglutamineorasparagine,argininecitrullinationandmethylation.C8-AandC8-BcontaineachtwomassisoformstermedC8-A(H),C8-A(L),C8-B(H)andC8-B(L),(H)standingforhigherand(L)forlowermolecularweight.C3,C4andC5arephosphorylated.Theratioofmethylatedarginineresiduesdecreasesinaging,makingtheproteinmorecationic.&TheN-terminalalanineisacetylated(isoform3,isoform4,isoform5andisoform6).&Arg-241wasfoundtobe6%monomethylatedand60%symmetricallydimethylated.DISEASE:SwissProt:P02686#Thereductioninthesurfacechargeofcitrullinatedand/ormethylatedMBPcouldresultinaweakenedattachmenttothemyelinmembrane.Thismechanismcouldbeoperativeindemyelinatingdiseasessuchaschronicalmultiplesclerosis(MS),andfulminatingMS(Marburgdisease).SIMILARITY:SwissProt:P02686##Belongstothemyelinbasicproteinfamily.MolecularWeight21,18,17&14kDa.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation