DescriptionCatalogueNumberMAB360Replaces04-1031;04-1062BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-GlialFibrillaryAcidicProteinAntibody,cloneGA5AlternateNamesGFAPBackgroundInformationGlialfibrillaryacidicproteinisaclass-IIIintermediatefilament.GFAPisthemainconstituentofintermediatefilamentsinastrocytesandservesasacellspecificMarkerthatdistinguishesdifferentiatedastrocytesfromotherglialcellsduringthedevelopmentofthecentralnervoussystem.ProductInformationFormatAscitesControlPositiveControl:Culturedastrocytes,braintissue(whitematter),spinalchord,retinaPresentationUnpurifiedmousemonoclonalIgG1liquidinbuffercontaining15mMsodiumazide.StorageandShippingInformationStorageConditionsStablefor1yearat-20ºCfromdateofreceipt.HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.ApplicationsApplicationAnti-GlialFibrillaryAcidicProteinAntibody,cloneGA5detectslevelofGlialFibrillaryAcidicProtein&hasbeenpublished&validatedforuseinIC,IH,IH(P)&WBwithmorethan65productcitations.KeyApplicationsImmunocytochemistryImmunohistochemistryImmunohistochemistry(Paraffin)WesternBlottingApplicationNotesImmunocytochemistry:Apreviouslotwasusedonculturedmammaliancells.Immunohistochemistry(paraffin):1:400-1:800dilutionfromapreviouslotwasusedonalcohol-fixedparaffinembeddedsectionsofratbrain(cerebrumorcerebellum)andhumanbrain.Immunoblotting:A1:1000dilutionofapreviouslotwasused.OptimalworkingdilutionsmustbedeterminedbytheenduserBIOLOGicalInformationImmunogenPurifiedGFAPfromporcinespinalcord.CloneGA5ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostMouseSpecificityGlialfibrillaracidicprotein.Onwesternblotsofextractsfromahumangliomacellline(U33CG/343MG),MAB360recognizesabandatapproximately51kDacorrespondingtoGFAP(Debus,1983).ByimmunohistochemistryitrecognizesastrocytesandBergmanngliacells,gliomaandglialcellderivedtumors.Showsnocross-reactivitywithvimentin.IsotypeIgG1SpeciesReactivityBovineChickenHumanMousePigRatRabbitSpeciesReactivityNoteReactswithBovine,Chicken,Human,Mouse,Porcine(Pig),RabbitandRat.Reactivitywithotherspecieshasnotbeendetermined.AntibodyTypeMonoclonalAntibodyEntrezGeneNumberNM_002055.2EntrezGeneSummaryThisgeneencodesoneofthemajorintermediatefilamentproteinsofmatureastrocytes.Itisusedasamarkertodistinguishastrocytesfromotherglialcellsduringdevelopment.MutationsinthisgenecauseAlexanderdisease,araredisorderofastrocytesinthecentralnervoussystem.Anadditionaltranscriptvarianthasbeendescribed,butitsfulllengthsequencehasnotbeendetermined.GeneSymbolGFAPFLJ45472PurificationMethodUnpurifiedUniProtNumberP14136UniProtSummaryFUNCTION:SwissProt:P14136#GFAP,aclass-IIIintermediatefilament,isacell-specificmarkerthat,duringthedevelopmentofthecentralnervoussystem,distinguishesastrocytesfromotherglialcells.SIZE:432aminoacids;49880DaSUBUNIT:Isoform3interactswithN-terminusofPSEN1.SUBCELLULARLOCATION:Cytoplasm.Note=Associatedwithintermediatefilaments.DISEASE:SwissProt:P14136#DefectsinGFAPareacauseofAlexanderdisease[MIM:203450].Alexanderdiseaseisararedisorderofthecentralnervoussystem.ItisaprogressiveleukoencephalopathywhosehallmarkisthewidespreadaccumulationofRosenthalfiberswhicharecytoplasmicinclusionsinastrocytes.Themostcommonformaffectsinfantsandyoungchildren,andischaracterizedbyprogressivefailureofcentralmyelination,usuallyleADIngtodeathusuallywithinthefirstdecade.InfantswithAlexanderdiseasedevelopaleukoencephalopathywithmacrocephaly,seizures,andpsychomotorretardation.Patientswithjuvenileoradultformstypicallyexperienceataxia,bulbarsignsandspasticity,andamoreslowlyprogressivecourse.SIMILARITY:SwissProt:P14136##Belongstotheintermediatefilamentfamily.MolecularWeight~51kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation