DescriptionCatalogueNumberHT17MG-14K-PX25TradeNameMILLIPLEXDescriptionMILLIPLEXMAPHumanTH17MagneticBeadPanelPremixed-25Plex-ImmunologyMultiplexAssayOverviewEMDMillipore’sMILLIPLEX®MAPHumanTH17PremixedMagneticBeadPanelisa25-plexkittobeusedforthesimultaneousquantificationofthefollowinganalytesinserum,plasmaorculturesupernatantsamples:GM-CSF,IFN-γ,IL-1β,IL-2,IL-4,IL-5,IL-6,IL-9,IL-10,IL-12(p70),IL-13,IL-15,IL-17A,IL-17F,IL-17E/IL-25,IL-21,IL-22,IL-23,IL-27,IL-28A,IL-31,IL-33,MIP-3α/CCL20,TNF-αandTNFβ.MILLIPLEX®MAPoffersthebroadestselectionofanalytesacrossawiderangeofdiseasestatesandspecies.Oncetheanalytesofinteresthavebeenidentified,youcanrelyonthequalitythatwebuildintoeachkittoproduceresultsyoucantrust.Inadditiontotheassaycharacteristicslistedintheprotocol,otherperformancecriteriaevaluatedduringthevalidationprocessinclude:cross-reactivity,dilutionlinearity,kitstABIlityandsamplebehavior(e.g.detectabilityandstability).Eachpanelandkitmeetsstringentmanufacturingcriteriatoensurebatch-to-batchreproducibility.EachMILLIPLEX®MAPpanelandkitincludes:•Qualitycontrols(QCs)providedtoqualifyassayperformance•Comparisonofstandard(calibrator)andQClotstoareferencelottoensurelot-to-lotconsistency•Optimizedserummatrixtomimicnativeanalyteenvironment•DetectionantibodycocktailsdesignedtoyieldconsistentanalyteprofileswithinpanelMILLIPLEX®MAPoffersyou:oTheabilitytochooseanycombinationofanalytesfromourpanelofsevenanalytestodesignacustomkitthatbettermeetsyourneeds.oAconvenient“all-in-one”boxformatthatgivesyoutheassurancethatyouwillhaveallthenecessaryreagentsyouneedtorunyourassay.EMDMillipore’sMILLIPLEX®MAPHumanTH17MagneticBeadPremixedPanelispartofthemostversatilesystemavailableforcytokineresearch.FromoursingletomultiplexbioMarkersolutions,wepartnerwithyoutodesign,develop,analyticallyvalidateandbuildthemostcomprehensivelibraryavailableforproteindetectionandquantitation.BackgroundInformationCD4T-helpercellsaremajorplayersinadaptiveimmunity.Basedontheirexpressionprofileoftranscriptionfactorsandsecretedcytokines,thesecellsaredividedintothemajorsubsetsTh1,Th2,Th17andTregulatory(Treg)cells.Th1cells,inducedbyIL-12andenhancedbyIFNγ,secreteIFNγthatactivatesmacrophages.InducedbyIL-4,Th2cellsprimarilyproduceIL-4,IL-5,IL-13,IL-25andIL-17E,playingaroleinallergy.Tregcells,inducedbyTGFβ,producetheanti-inflammatorycytokinesIL-10andTGFβandfunctiontocontrolT-cellresponsestopreventautoimmunereactions.Th17cells,amorerecentlydiscoveredsubsetcharacterizedbytheproductionofIL-17,areinducedbyTGFβcombinedwithIL-23andIL-1βinhumans,andTGFβ,IL-6andIL-21inmice.ActivatedTh17cellssecreteIL-17A,IL-17F,IL-21,IL-22andTNFαtopromotetissueinflammation.Th17cellsareinvolvedintheclearanceofextracellularbacteriaandfungi.TheyareabundantintheintestinallaminapropriaandfunctionasabarrieragainstinvADIngpathogens.ExcessiveamountsofTh17cellshavebeenimplicatedinthepathogenesisofseveralautoimmunediseases,includingmultiplesclerosis,psoriasis,juvenilediabetes,rheumatoidarthritis,Crohn"sdiseaseandautoimmuneuveitis.Inaddition,Th17cellsplayanimportantroleintumordevelopment,progressionandmetastasis,potentiallyinbothpromotingandinhibitingtumorgrowth.TheMILLIPLEX®MAPHumanTH17MagneticBeadPanelenablesyoutofocusontherelevanceandtherapeuticpotentialofthemodulationofCD4T-helpercellcytokinesinyourresearchmodel.CoupledwiththeLuminexxMAP®platforminamagneticbeadformat,youreceivetheadvantageofidealspeedandsensitivity,allowingquantitativemultiplexdetectionofmultipleanalytessimultaneously,whichcandramaticallyimproveproductivity.ProductInformationDetectionmethodLuminexxMAPConfigurationPremixedPrecision,%Inter-assay:Intra-assay:PanelTypeMAGNETICCytokines/ChemokinesStorageandShippingInformationStorageConditionsRecommendedstorageforkitcomponentsis2-8°C.ApplicationsApplicationSimultaneouslyanalyzemultipleTh17cytokineandchemokinebiomarkerswiththeTh17Bead-BasedMultiplexAssaysusingtheLuminextechnology,inhumanserum,plasmaandcellculturesamples.KeyApplicationsMultiplexingApplicationNotes•Thisisanovernightincubationassayrequiring25µLofneatplasmaorserum,or25μLcellculturesupernatantperwell.•ThiskitmaybeusedfortheanalysisofalloranycombinationoftheTh17panelanalytesinserum,plasmaortissue/celllysateandculturesupernatantBIOLOGicalInformationSpeciesReactivityHumanAnalytesAvailableGM-CSFIFN-γIL-1βIL-2IL-4IL-5IL-6IL-9IL-10IL-12(p70)IL-13IL-15IL-17AIL-17FIL-17E/IL-25IL-21IL-22IL-23IL-27IL-28AIL-31IL-33MIP-3α/CCL20TNF-αTNFβCrossReactivityCross-reactivitybetweentheantibodiesandanyoftheotheranalytesinthispanelisnon-detectableornegligIBLe.Accuracy79-107%PhysicochemicalInformationSensitivityRefertokitprotocolforsensitivitiesofindividualbiomarkers.StandardCurveRangeIL-17&IL-4,24-100,000pg/mLGM-CSF&IL-27,61-250,000pg/mLIFNγ,10-40,000pg/mLIL-10,1-5000pg/mLMIP-3α,IL-1β,IL-12(p70),IL-15,IL-21&IL-33,5-20,000pg/mLIL-13,7-30,000pg/mLIL-17A&IL-2,12-50,000pg/mLIL-22&TNFβ,37-150,000pg/mLIL-9,9-35,000pg/mLIL-23,336-1,500,000pg/mLIL-5,6-25,000pg/mLIL-6&TNFα,3-10,000pg/mLIL-17E/IL-25,488-2,000,000IL-28a&IL-31,49-200,000pg/mLDimensionsMaterialsInformationMaterialsInformation