DescriptionCatalogueNumberMTH17MAG-47KTradeNameMILLIPLEXDescriptionMILLIPLEXMAPMouseTH17MagneticBeadPanel-ImmunologyMultiplexAssayOverviewTheMILLIPLEXMAPMouseTh17MagneticBeadpanelenablesyoutofocusonthetherapeuticpotentialofthemodulationofCD4T-helpercellcytokines.CoupledwiththeLuminex®xMAP®platforminamagneticbeadformat,youreceivetheadvantageofidealspeedandsensitivity,allowingquantitativemultiplexdetectionofdozensofanalytessimultaneously,whichcandramaticallyimproveproductivity.BackgroundInformationCD4T-helpercellsaremajorplayersinadaptiveimmunity.Basedontheirexpressionprofileoftranscriptionfactorsandsecretedcytokines,thesecellsaredividedintothemajorsubsetsTh1,Th2,Th17andTregulatory(Treg)cells.Th1cells,inducedbyIL-12andenhancedbyIFN,secreteIFNthatactivatesmacrophages.InducedbyIL-4,Th2cellsprimarilyproduceIL-4,IL-5,IL-13,IL-25andIL-17E,playingaroleinallergy.Tregcells,inducedbyTGFβ,producetheanti-inflammatorycytokinesIL-10andTGFβandfunctiontocontrolT-cellresponsestopreventautoimmunereactions.Th17cells,amorerecentlydiscoveredsubsetcharacterizedbytheproductionofIL-17,areinducedbyTGFβcombinedwithIL-23andIL-1βinhumans,andTGFβ,IL-6andIL-21inmice.ActivatedTh17cellssecreteIL-17A,IL-17F,IL-21,IL-22andTNFαtopromotetissueinflammation.Th17cellsareinvolvedintheclearanceofextracellularbacteriaandfungi.TheyareabundantintheintestinallaminapropriaandfunctionasabarrieragainstinvADIngpathogens.ExcessiveamountsofTh17cellshavebeenimplicatedinthepathogenesisofseveralautoimmunediseases,includingmultiplesclerosis,psoriasis,juvenilediabetes,rheumatoidarthritis,Crohn"sdiseaseandautoimmuneuveitis.InadditionTh17cellsplayanimportantroleintumordevelopment,progressionandmetastasis,potentiallyinbothpromotingandinhibitingtumorgrowth.ProductInformationDetectionmethodLuminexxMAPConfigurationDesignyourmultiplexkitbychoosingavailableanalyteswithinthispanel.Precision,%Inter-assay:Intra-assay:PanelTypeMAGNETICCytokines/ChemokinesStorageandShippingInformationStorageConditionsRecommendedstorageforkitcomponentsis2-8°C.ApplicationsApplicationSimultaneouslyanalyzemultipleTh17cytokineandchemokinebioMarkerswiththeTh17Bead-BasedMultiplexAssaysusingtheLuminextechnology,inhumanserum,plasmaandcellculturesamples.KeyApplicationsMultiplexingApplicationNotes•Thisisatwo-hourorovernightincubationassayrequiring25µLofneatplasmaorserum,or25μLcellculturesupernatantperwell.•ThiskitmaybeusedfortheanalysisofalloranycombinationoftheTh17panelanalytesinserum,plasmaortissue/celllysateandculturesupernatantBIOLOGicalInformationSpeciesReactivityMouseAnalytesAvailableCD40LigandGM-CSFIFN-γIL-1βIL-2IL-4IL-5IL-6IL-10IL-12(p70)IL-13IL-15IL-17AIL-17E/IL-25IL-17FIL-21IL-22IL-23IL-27IL-28BIL-31IL-33MIP-3α/CCL20TNF-αTNFβCrossReactivityTherewasnoorminimumcross-reactivitybetweentheantibodiesandanyotheranalytesinthepanel(seedetailsinassayprotocol).PhysicochemicalInformationSensitivityRefertokitprotocolforsensitivitiesofindividualbiomarkers.LinearityofDilution1:4111.0%1:8113.1%StandardCurveRangeCD40LIL-31&MIP-3α:49–50,000pg/mLGM-CSF&IL-15:34–35,000pg/mLIFNγ&IL-6:7.8–8,000pg/mLIL-1β:15–15,000pg/mLIL-2:6.9–6,000pg/mLIL-4:1.5–1,500pg/mLIL-5:4.9–5,000pg/mLIL-10,IL-12(p70),&IL-21:20–20,000pg/mLIL-13&IL-17A:39–40,000pg/mLIL-17E/IL-25:586–600,000pg/mLIL-17F:10–10,000pg/mLIL-22:2.4–2,500pg/mLIL-23:342-350,000pg/mLIL-27:879–900,000pg/mLIL-28B:127–130,000pg/mLIL-33:78–80,000pg/mLTNFβ:488–500,000pg/mLTNFα:3.4–3,500pg/mLDimensionsMaterialsInformationMaterialsInformation