DescriptionCatalogueNumberAB1846BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-C-X-CChemokineReceptor4Antibody,NTAlternateNamesCD184CXCR4ProductInformationFormatPurifiedControlSkinPresentationPurifiedIgGinPBScontaining0.02%sodiumazide.StorageandShippingInformationStorageConditionsMaintain1yearat4°C,fromdateofshipment.Donotfreeze.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ApplicationsApplicationAnti-C-X-CChemokineReceptor4Antibody,N-terminusisanantibodyagainstC-X-CChemokineReceptor4foruseinIC,IP&WB.KeyApplicationsImmunocytochemistryImmunoprecipitationWesternBlottingApplicationNotesWesternblot:1-2μg/mL.HeLacelllysatescanbeusedaspositivecontrol.Theantibodyshoulddetectabandat40kDa.Immunocytochemistry:10μg/mLImmunoprecipitationOptimalworkingdilutionsmustbedeterminedbyenduser.BIOLOGicalInformationImmunogenAmino-terminuspeptideofhumanCXCR4(Berson,1996;Leotscher,1994).EpitopeN-terminusConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostRabbitSpecificityRecognizesCXCR4,N-terminus.SpeciesReactivityHumanAntibodyTypePolyclonalAntibodyEntrezGeneNumberNM_001008540.1NM_003467.2EntrezGeneSummaryThisgeneencodesaCXCchemokinereceptorspecificforstromalcell-derivedfactor-1.Theproteinhas7transmembraneregionsandislocatedonthecellsurface.ItactswiththeCD4proteintosupportHIVentryintocellsandisalsohighlyexpressedinbreastcancercells.MutationsinthisgenehavebeenassociatedwithWHIM(warts,hypogammaglobulinemia,infections,andmyelokathexis)syndrome.Alternatetranscriptionalsplicevariants,encodingdifferentisoforms,havebeencharacterized.GeneSymbolCXCR4WHIMNPY3RNPYY3RFB22LAP3FusinCXCR-4LCR1NPYRHM89fusinHSY3RRCXC-R4NPYY3NPYRLD2S201ECD184LESTRPurificationMethodProteinAPurfiedUniProtNumberP61073UniProtSummaryFUNCTION:SwissProt:P61073#ReceptorfortheC-X-CchemokineCXCL12/SDF-1.Transducesasignalbyincreasingtheintracellularcalciumionslevel.Involvedinhaematopoiesisandincardiacventricularseptumformation.Playsalsoanessentialroleinvascularizationofthegastrointestinaltract,probablybyregulatingvascularbranchingand/orremodelingprocessesinendothelialcells.Couldbeinvolvedincerebellardevelopment.IntheCNS,couldmediatehippocampal-neuronsurvival.Actsasacoreceptor(CD4beingtheprimaryreceptor)forHIV-1X4isolatesandasaprimaryreceptorforsomeHIV-2isolates.PromotesEnv-mediatedfusionofthevirus.SIZE:352aminoacids;39746DaSUBUNIT:InteractswithHIV-1surfaceproteingp120andTat.SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.TISSUESPECIFICITY:Expressedinnumeroustissues,suchasperipheralbloodleukocytes,spleen,thymus,spinalcord,heart,placenta,lung,liver,skeletalmuscle,kidney,pancreas,cerebellum,cerebralcortexandmedulla(inmicrogliaaswellasinastrocytes),brainmicrovascular,coronaryarteryandumbilicalcordendothelialcells.Isoform1ispredominantinalltissuestested.DOMAIN:SwissProt:P61073Theamino-terminusiscriticalforligandbinding.ResiduesinallfourextracellularregionscontributetoHIV-1coreceptoractivity.PTM:Sulfated.DISEASE:SwissProt:P61073#DefectsinCXCR4areacauseofWHIMsyndrome[MIM:193670];alsocalledwarts,hypogammaglobulinemia,infections,andmyelokathexis.WHIMsyndromeisanimmunodeficiencydiseasecharacterizedbyneutropenia,hypogammaglobulinemiaandextensivehumanpapillomavirus(HPV)infection.Despitetheperipheralneutropenia,bonemarrowaspiratesfromaffectedindividualscontainabundantmaturemyeloidcells,aconditiontermedmyelokathexis.SIMILARITY:BelongstotheG-proteincoupledreceptor1family.MolecularWeight40kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation