DescriptionCatalogueNumberAB4250BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-CXCChemokineReceptor3Antibody,CTdomainAlternateNamesCXCR3BackgroundInformationCXCR3,achemokinereceptor,ishighlysimilartotheinterleukin8BreceptorandhasbeenshowntomediaterecruitmentofTcellstositesofinflammationandretentionofT-cellsintheinflamedCNS.CXCR3hasbeensuggestedtobetheprincipalinflammatorychemokinereceptorinvolvedinintrathecalaccumulationofT-cellsinmultiplesclerosisanddiabetes.ItisexpressedonmalignantBcellsinchroniclymphoproliferativedisorders(Jonesetal.,2000).Analternativelysplicedisoform,CXCR3B,hasrecentlybeenidentified(Lasagnietal.,2003).CXCR3expressionhasbeendocumentedinthebrain,lymphoidandotherperipheraltissues,andindiseasesincludingatherosclerosis,Alzheimer"s,multiplesclerosis,rheumatoidarthritis,lymphomas,asthma,neoplasms,leukemia,andinflammatoryconditionsofvariousorgans.ProductInformationFormatAffinityPurifiedPresentationPeptide-affinitypurifiedimmunoglobulin.Liquidinphosphatebufferedsaline,pH7.7,containing0.01%sodiumazideStorageandShippingInformationStorageConditionsMaintainat-70°Cinundilutedaliquotsforupto12monthsfromdateofreceipt.Maybestoredat2-8°Cforshorttermuse.Avoidrepeatedfreeze/thawcycles.ApplicationsApplicationAnti-CXCChemokineReceptor3Antibody,C-terminaldomaindetectslevelofCXCChemokineReceptor3&hasbeenpublished&validatedforuseinIH(P).KeyApplicationsImmunohistochemistry(Paraffin)ApplicationNotesImmunohistochemistry(formalin-fixedparaffin-embeddedtissues)Optimalworkingdilutionsmustbedeterminedbytheenduser.BIOLOGicalInformationImmunogenSyntheticpeptidecorrespondingtoasequencewithintheC-terminaldomainofhumanCXCR3.Theimmunogenhas82%homologywithratandmouse.EpitopeC-terminaldomainConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostRabbitSpecificityRecognizeshumanCXCR3.ThespecificityofthisantibodyhasbeenevaluatedbyIHCstainingofhumantonsil.SpeciesReactivityHumanAntibodyTypePolyclonalAntibodyEntrezGeneNumberNM_001504.1EntrezGeneSummaryCD183isaGprotein-coupledreceptorwithselectivityforthreechemokines,termedIP10(interferon-g-inducIBLe10kDaprotein),Mig(monokineinducedbyinterferon-g)andI-TAC(interferon-inducibleTcella-chemoattractant).IP10,MigandI-TACbelongtothestructuralsubfamilyofCXCchemokines,inwhichasingleaminoacidresidueseparatesthefirsttwooffourhighlyconservedCysresidues.Historically,CD183isthethirdCXCchemokinereceptordiscoveredand,therefore,commonlydesignatedasCXCR3.BindingofchemokinestoCD183inducescellularresponsesthatareinvolvedinleukocytetraffic,mostnotablyintegrinactivation,cytoskeletalchangesandchemotacticmigration.InhibitionbyBordetellapertussistoxinsuggeststhatheterotrimericGproteinoftheGi-subclasscoupletoCD183.Signaltransductionhasnotbeenfurtheranalyzedbutmayincludethesameenzymesthatwereidentifiedinthesignalingcascadeinducedbyotherchemokinereceptors.Asaconsequenceofchemokine-inducedcellulardesensitization(phosphorylation-dependentreceptorinternalization),cellularresponsesaretypicallyrapidandshortinduration.Cellularresponsivenessisrestoredafterdephosphorylationofintracellularreceptorsandsubsequentrecyclingtothecellsurface.AhallmarkofCD183isitsprominentexpressionininvitroculturedeffector/memoryTcells,andinTcellspresentinmanytypesofinflamedtissues.Inaddition,IP10,MigandI-TACarecommonlyproducedbylocalcellsininflammatorylesion,suggestingthatCD183anditschemokinesparticipateintherecruitmentofinflammatorycells.Therefore,CD183isatargetforthedevelopmentofsmallmolecularweightantagoNISTs,whichmaybeusedinthetreatmentofdiverseinflammatorydiseases.GeneSymbolCXCR3CD182CXC-R3IP10-RIP10Mig-RCD183CKR-L2CMKAR3GPR9CXCR-3MigRUniProtNumberP49682UniProtSummaryFUNCTION:SwissProt:P49682#ReceptorforCXCL9,CXCL10andCXCL11andmediatestheproliferationofhumanmesangialcells(HMC).Isoform2isareceptorforCXCL4andalsomediatestheinhibitoryactivitiesofCXCL9,CXCL10andCXCL11onthegrowthofhumanmicrovascularendothelialcells(HMVEC).Isoform2mayplayaroleinangiogenesis.Isoform3mediatesactivityofCXCL11.SIZE:368aminoacids;40660DaSUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.TISSUESPECIFICITY:Isoform1andisoform2aremainlyexpressedinheart,kidney,liverandskeletalmuscle.Isoform1isalsoexpressedinplacenta.SIMILARITY:SwissProt:P49682##BelongstotheG-proteincoupledreceptor1family.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation