DescriptionCatalogueNumberAB4251BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-CXCChemokineReceptor4Antibody,NT,extracellularAlternateNamesCXCR4BackgroundInformationCXCR4isachemokinereceptorinvolvedinorganvascularization,neuronalcellmigration,andpatterningofthecentralnervoussystemduringdevelopment.Itbindsstromalcell-derivedfactor1(SDF1)andmediatesmigrationofrestingleukocytesandisuniqueinhominghematopoieticProgenitorstobonemarrow.ThesepropertiessuggestthatCXCR4isinvolvedintumorcellmigrationandlocaltumorinvasion.ItisalsoanHIV-1fusionco-factorthatallowsHIV-1invasionindiversehumancelltypes.CXCR4hastwoisoformsthatareproducedbyalternativesplicingandhasbeenreportedtobeexpressedincellsoftheimmuneandcentralnervoussystems.Itishighlyexpressedinbrainandheart,andinwhitebloodcells,vascularendothelialcells,andumbilicalcordendothelialcells.Inbraintumors,CXCR4isexpressedinregionsofangiogenesisanddegenerative,necrotic,andmicrocysticchanges(Rempeletal.,2000).ESTshavebeenisolatedfromawidevarietyoftissuelibraries,particularlyuterus,kidney,lung,tonsil,andbrain.ProductInformationFormatAffinityPurifiedPresentationPeptide-affinitypurifiedimmunoglobulin.Liquidinphosphatebufferedsaline,pH7.7,containing0.01%sodiumazideStorageandShippingInformationStorageConditionsMaintainat-70°Cinundilutedaliquotsforupto12monthsfromdateofreceipt.Maybestoredat2-8°Cforshorttermuse.Avoidrepeatedfreeze/thawcycles.ApplicationsApplicationThisAnti-CXCChemokineReceptor4Antibody,N-terminal,extracellularisvalidatedforuseinIH(P)forthedetectionofCXCChemokineReceptor4.KeyApplicationsImmunohistochemistry(Paraffin)ApplicationNotesImmunohistochemistry(formalin-fixedparaffin-embeddedtissues)Optimalworkingdilutionsmustbedeterminedbytheenduser.BIOLOGicalInformationImmunogenSyntheticpeptidecorrespondingtoasequencewithintheN-terminalextracellulardomainofhumanCXCR4.Theimmunogenhas93%homologywithmouseandrat.EpitopeN-terminal,extracellularConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostRabbitSpecificityRecognizeshumanCXCR4.ThespecificityofthisantibodyhasbeenconfirmedbyIHCstainingofcardiacmyocytesinhumanheart.SpeciesReactivityHumanAntibodyTypePolyclonalAntibodyEntrezGeneNumberNM_001008540.1NM_003467.2EntrezGeneSummaryThisgeneencodesaCXCchemokinereceptorspecificforstromalcell-derivedfactor-1.Theproteinhas7transmembraneregionsandislocatedonthecellsurface.ItactswiththeCD4proteintosupportHIVentryintocellsandisalsohighlyexpressedinbreastcancercells.MutationsinthisgenehavebeenassociatedwithWHIM(warts,hypogammaglobulinemia,infections,andmyelokathexis)syndrome.Alternatetranscriptionalsplicevariants,encodingdifferentisoforms,havebeencharacterized.GeneSymbolCXCR4WHIMNPY3RNPYY3RFB22LAP3FusinCXCR-4LCR1NPYRHM89fusinHSY3RRCXC-R4NPYY3NPYRLCD184D2S201ELESTRUniProtNumberP61073UniProtSummaryFUNCTION:SwissProt:P61073#ReceptorfortheC-X-CchemokineCXCL12/SDF-1.Transducesasignalbyincreasingtheintracellularcalciumionslevel.Involvedinhaematopoiesisandincardiacventricularseptumformation.Playsalsoanessentialroleinvascularizationofthegastrointestinaltract,probablybyregulatingvascularbranchingand/orremodelingprocessesinendothelialcells.Couldbeinvolvedincerebellardevelopment.IntheCNS,couldmediatehippocampal-neuronsurvival.Actsasacoreceptor(CD4beingtheprimaryreceptor)forHIV-1X4isolatesandasaprimaryreceptorforsomeHIV-2isolates.PromotesEnv-mediatedfusionofthevirus.SIZE:352aminoacids;39746DaSUBUNIT:InteractswithHIV-1surfaceproteingp120andTat.SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.TISSUESPECIFICITY:Expressedinnumeroustissues,suchasperipheralbloodleukocytes,spleen,thymus,spinalcord,heart,placenta,lung,liver,skeletalmuscle,kidney,pancreas,cerebellum,cerebralcortexandmedulla(inmicrogliaaswellasinastrocytes),brainmicrovascular,coronaryarteryandumbilicalcordendothelialcells.Isoform1ispredominantinalltissuestested.DOMAIN:SwissProt:P61073Theamino-terminusiscriticalforligandbinding.ResiduesinallfourextracellularregionscontributetoHIV-1coreceptoractivity.PTM:Sulfated.DISEASE:SwissProt:P61073#DefectsinCXCR4areacauseofWHIMsyndrome[MIM:193670];alsocalledwarts,hypogammaglobulinemia,infections,andmyelokathexis.WHIMsyndromeisanimmunodeficiencydiseasecharacterizedbyneutropenia,hypogammaglobulinemiaandextensivehumanpapillomavirus(HPV)infection.Despitetheperipheralneutropenia,bonemarrowaspiratesfromaffectedindividualscontainabundantmaturemyeloidcells,aconditiontermedmyelokathexis.SIMILARITY:BelongstotheG-proteincoupledreceptor1family.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation