DescriptionCatalogueNumberHTS002MBrandFamilyChemicon®TradeNameChemiScreenChemiconDescriptionChemiScreen™CXCR2MembranePreparationOverviewTRANSFECTION:Full-lengthhumanCXCR2CDNA(AccessionNumber:M73969)BackgroundInformationCXCR2isa7-TMG-proteincoupledreceptorthatbindstothechemokinesGROalpha,GRObeta,GROgamma,IL-8,ENA-78,NAP-2andGCP-2(OlsonandLey,2002).Neutrophils,mastcellsandmicrovascularendothelialcellsexpressCXCR2,andinteractionsofCXCR2withitsligandspromoteschemotaxisofthesecelltypes(Heidemannetal.,2003;Nilssonetal.,1999;Whiteetal.,1998).StudieswithmicelackingCXCR2indicatethatCXCR2promotesgrowthofprimarytumorsandsecondarymetastases(Keaneetal.,2004),andplaysanessentialroleinhyperoxia-inducedlunginjury(Sueetal.,2004).Inaddition,cytomegalovirusencodesaCXCR2-bindingchemokine,vCXC-1,thatpromotesneutrophilmigrationtoinfectedcells(Penfoldetal.,1999).Chemicon"sCXCR2MembranePrepsareidealtoolsforscreeningforantagoNISTsofinteractionsbetweenCXCR2anditsligands.RADIolabeledIL-8bindstotheCXCR2membraneswithaKdof0.075nM.With0.1nM125I-labeledIL-8,a5-foldwindowofspecificbindingisobtained.ProductInformationFormatMembranesPresentationMembraneswereadjustedto0.5mgprotein/mlin50mMTrispH7.4,10%glyceroland1%BSA,rapidlyfrozen,andstoredat-80°C.Onevialtypicallycontains1000units,whereaunitistheamountofmembranethatwillyieldgreaterthan1000cpmspecificbindingof125I-labeledIL-8(0.1nMinputconcentration).StorageandShippingInformationStorageConditionsMaintainfrozenat-70°Cforupto2years.Donotfreezeandthaw.ApplicationsApplicationHumanCXCR2/IL-8bGPCRmembranepreparationforRadioligandbindingAssays.KeyApplicationsRadioligandbindingassayApplicationNotesRadioligandbindingassay.BIOLOGicalInformationConcentration0.5mg/mLEntrezGeneNumberNM_001557.2EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheG-protein-coupledreceptorfamily.Thisproteinisareceptorforinterleukin8(IL8).ItbindstoIL8withhighaffinity,andtransducesthesignalthroughaG-proteinactivatedsecondmessengersystem.Thisreceptoralsobindstochemokine(C-X-Cmotif)ligand1(CXCL1/MGSA),aproteinwithmelanomagrowthstimulatingactivity,andhasbeenshowntobeamajorcomponentrequiredforserum-dependentmelanomacellgrowth.Thisreceptormediatesneutrophilmigrationtositesofinflammation.TheangiogeniceffectsofIL8inintestinalmicrovascularendothelialcellsarefoundtobemediatedbythisreceptor.Knockoutstudiesinmicesuggestedthatthisreceptorcontrolsthepositioningofoligodendrocyteprecursorsindevelopingspinalcordbyarrestingtheirmigration.Thisgene,IL8RA,ageneencodinganotherhighaffinityIL8receptor,aswellasIL8RBP,apseudogeneofIL8RB,formageneclusterinaregionmappedtochromosome2q33-q36.GeneSymbolIL8RBCD182CMKAR2CXCR-2IL8RAIL8R2CXCR2CDw128bGPCRClassAHostCellsChem-1ProteinTargetCXCR2/IL-8bTargetSub-FamilyChemokineUniProtNumberP25025UniProtSummaryFUNCTION:SwissProt:P25025#Receptortointerleukin-8,whichisapowerfulneutrophilschemotacticfactor.BindingofIL-8tothereceptorcausesactivationofneutrophils.ThisresponseismediatedviaaG-proteinthatactivateaphosphatidylinositol-calciumsecondmessengersystem.ThisreceptorbindstoIL-8withahighaffinityandtoGRO/MGSAandNAP-2alsowithahighaffinity.SIZE:360aminoacids;40759DaSUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.PTM:Phosphorylateduponligandbinding;whichisrequiredfordesensitization.SIMILARITY:SwissProt:P25025##BelongstotheG-proteincoupledreceptor1family.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation