DescriptionCatalogueNumberAB1847BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-C-X-CChemokineReceptor4Antibody,extracellularloopAlternateNamesCD184CXCR4ProductInformationFormatPurifiedPresentationPurifiedIgGinPBScontaining0.02%sodiumazide.StorageandShippingInformationStorageConditionsCanberefrigeratedat2-8°Cinundilutedaliquotsforupto12monthsfromdateofreceipt.ApplicationsApplicationAnti-C-X-CChemokineReceptor4Antibody,extracellularloopdetectslevelofC-X-CChemokineReceptor4&hasbeenpublished&validatedforuseinWB.KeyApplicationsWesternBlottingApplicationNotesWesternblot:1:500-1000Optimalworkingdilutionsmustbedeterminedbyenduser.BIOLOGicalInformationImmunogenExtracellularlooppeptideofhumanCXCR4(Bersonetal.1996;Leotscheretal.1994).EpitopeextracellularloopHostRabbitSpecificitySomeGprotein-coupledreceptorsincludingCCR5,CXCR4,CCR3,CCR2bandCCR8inthechemokinereceptorfamily,andfournewhumanmoleculesGPR15,STRL33,GPR1andV28wererecentlyidentifiedasHIVcoreceptors(Nimitrov1997).Amongthem,CXCR4(fusin,LESTRorHUMSTR)isaprinciplecoreceptorforT-celltropicstrainsofHIV-1fusionandentryofhumanwhitebloodcells(Fengetal.1996;Bersonetal.1996).CXCR4isalsorequiredfortheinfectionbydual-tropicstrainsofHIV-1andmediatesCD-4independentinfectionbyHIV-2(Doranzetal.1996;Endresetal.1996).Thea-chemokineSDF-1istheligandforCXCR4andpreventsinfectionbyT-tropicHIV-1(Bleuletal.1996;Oberlinetal.1996).CXCR4associateswiththesurfaceCD4-gp120complexbeforeHIVenterstargetcells(Laphametal.1996).CXCR4messengerRNAlevelscorrelatedwithHIV-1permissivenessindiversehumancelltypes(Fengetal.1996).AntibodiestoCXCR4blockHIV-1andHIV-2fusionandinfectionofhumantargetcells(Fengetal.1996;Endresetal.1996;Brelotetal.1997).Theamino-terminaldomainandthesecondextracellularloopofCXCR4serveasHIVbindingsites(Brelotetal.1997;Luetal.1997).SpeciesReactivityHumanMouseAntibodyTypePolyclonalAntibodyEntrezGeneNumberNM_001008540.1NM_003467.2EntrezGeneSummaryThisgeneencodesaCXCchemokinereceptorspecificforstromalcell-derivedfactor-1.Theproteinhas7transmembraneregionsandislocatedonthecellsurface.ItactswiththeCD4proteintosupportHIVentryintocellsandisalsohighlyexpressedinbreastcancercells.MutationsinthisgenehavebeenassociatedwithWHIM(warts,hypogammaglobulinemia,infections,andmyelokathexis)syndrome.Alternatetranscriptionalsplicevariants,encodingdifferentisoforms,havebeencharacterized.GeneSymbolCXCR4WHIMNPY3RNPYY3RFB22LAP3FusinCXCR-4LCR1NPYRHM89fusinHSY3RRCXC-R4NPYY3NPYRLD2S201ECD184LESTRUniProtNumberP61073UniProtSummaryFUNCTION:SwissProt:P61073#ReceptorfortheC-X-CchemokineCXCL12/SDF-1.Transducesasignalbyincreasingtheintracellularcalciumionslevel.Involvedinhaematopoiesisandincardiacventricularseptumformation.Playsalsoanessentialroleinvascularizationofthegastrointestinaltract,probablybyregulatingvascularbranchingand/orremodelingprocessesinendothelialcells.Couldbeinvolvedincerebellardevelopment.IntheCNS,couldmediatehippocampal-neuronsurvival.Actsasacoreceptor(CD4beingtheprimaryreceptor)forHIV-1X4isolatesandasaprimaryreceptorforsomeHIV-2isolates.PromotesEnv-mediatedfusionofthevirus.SIZE:352aminoacids;39746DaSUBUNIT:InteractswithHIV-1surfaceproteingp120andTat.SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.TISSUESPECIFICITY:Expressedinnumeroustissues,suchasperipheralbloodleukocytes,spleen,thymus,spinalcord,heart,placenta,lung,liver,skeletalmuscle,kidney,pancreas,cerebellum,cerebralcortexandmedulla(inmicrogliaaswellasinastrocytes),brainmicrovascular,coronaryarteryandumbilicalcordendothelialcells.Isoform1ispredominantinalltissuestested.DOMAIN:SwissProt:P61073Theamino-terminusiscriticalforligandbinding.ResiduesinallfourextracellularregionscontributetoHIV-1coreceptoractivity.PTM:Sulfated.DISEASE:SwissProt:P61073#DefectsinCXCR4areacauseofWHIMsyndrome[MIM:193670];alsocalledwarts,hypogammaglobulinemia,infections,andmyelokathexis.WHIMsyndromeisanimmunodeficiencydiseasecharacterizedbyneutropenia,hypogammaglobulinemiaandextensivehumanpapillomavirus(HPV)infection.Despitetheperipheralneutropenia,bonemarrowaspiratesfromaffectedindividualscontainabundantmaturemyeloidcells,aconditiontermedmyelokathexis.SIMILARITY:BelongstotheG-proteincoupledreceptor1family.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation