首页 > Millipore > Millipore/HTS055M | ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor/HTS055M/200 units

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

Millipore/HTS055M | ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor/HTS055M/200 units

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

HTS055M

品牌：

Millipore

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

DescriptionCatalogueNumberHTS055MBrandFamilyChemicon®TradeNameChemiScreenChemiconDescriptionChemiSCREEN™MembranePreparationRecombinantHumanCXCR5ChemokineReceptorOverviewHumanCXCR5BackgroundInformationCXCR5(alsoknownasBLR1)isaGPCRthatbindsselectivelytothechemokineCXCL13(alsoknownasBLCandBCA-1)tomediateimmunesystemdevelopmentandfunction(Försteretal.,1996;Gunnetal.,1998).CXCR5cooperateswithCCR7todeterminefunctionalorganizationoflymphoidorganssuchaslymphnodesandPeyer’spatches(Ohletal.,2003).Inaddition,CXCR5mediatesmigrationofBcellsintothefolliclesofthesplenicwhitepulp,thuspermittingantigendepositiononfolliculardendriticcells(Försteretal.,1996;Cinamonetal.,2008).Patientswithrheumatoidarthritis(RA)displayedupregulatedexpressionofCXCR5toagreaterextentthanotherchemokinereceptors,andCXCR5-nullmicesubjectedtoantigen-inducedarthritisdisplayreducedjointdestruction(Schmutzetal,2004;Wengneretal,2007).Therefore,CXCR5representsapotentialtargetfortreatmentofrheumatoidarthritis.Millipore"sCXCR5MembranePreparationforGTPγSAssayisacrudemembranepreparationmadefromtheproprietarystablerecombinantcelllinethatisalsousedforMillipore’sCXCR5CalciumOptimizedCellLine(HTS055C).ThisCXCR5MembranePreparationhasbeenoptimizedforlowbasalactivityinGTPγSbindingassays;thusitisanidealHTStoolforscreeningofcompoundsforagoNISTandantagonistactivities.ThemembranepreparationsexhibitsEC50of1.8nMforCXCL13inaGTPγSbindingassay.ProductInformationFormatMembranesPresentationLiquidinpackagingbuffer:50mMTrispH7.4,10%glyceroland1%BSAwithnopreservatives.Packagingmethod:Membraneproteinwasadjustedtotheindicatedconcentrationinpackagingbuffer,rapidlyfrozen,andstoredat-80°C.StorageandShippingInformationStorageConditionsStoreat–70°C.Productisstableforatleast6monthsfromthedateofreceiptwhenstoredasdirected.Donotfreezeandthaw.ApplicationsApplicationHumanCXCR5GPCRmembranepreparationforRADIoligandbindingAssays>PγSbinding.KeyApplicationsRadioligandbindingassayGTPγSbindingApplicationNotesRadioligandbindingassayandGTPγSbindingBIOLOGicalInformationEntrezGeneNumberNM_001716GeneSymbolCXCR5BLR1MDR15GPCRClassAHostCellsChem-5ProteinTargetCXCR5TargetSub-FamilyChemokineUniProtNumberP32302IncubationConditionsMembranesarepermeABIlizedbyadditionofsaponintoanequalconcentrationbymass,thenmixedwith[35S]-GTPγS(finalconcentrationof0.3nM)in20mMHEPES,pH7.4/100mMNaCl/10mMMgCl2/0.5μMGDPinanonbinding96-wellplate.UnlabeledCXCL13wasaddedtothefinalconcentrationindicatedinFigure1(finalvolume100μL),andincubatedfor30minat30°C.ThebindingreactionistransferredtoanFBfilterplate(MilliporeMAHFB1H)previouslyprewettedwithwater.Theplateiswashed3times(1mLperwellperwash)withcold10mMsodiumphosphate,pH7.4,thendriedandcounted.Onepackagecontainsenoughmembranesforatleast200assays(units),whereoneunitistheamountofmembranethatwillyieldgreaterthan1000cpmspecificCXCL13-stimulated[35S]-GTPγSbinding.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation

密理博(Millipore)公司成立于1954年，总部位于美国麻省，在全世界设有47个办事处，为100多个国家提供产品和技术服务。目前全球雇员超过5800人，在美国、法国和日本等国家拥有7家大型生产工厂，主要生产过滤膜及膜过滤产品。

20世纪80年代，密理博公司进入中国市场。先后在香港、北京、上海、广州及成都设立了办事机构，并于2000年4月在上海浦东外高桥保税区建立了密理博（上海）贸易有限公司。

为了更好地满足中国用户的需求，密理博中国主页于2006年11月向广大用户开放，介绍密理博中国有限公司的最新动态，力求为用户打造专业的产品与服务信息交流平台。