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QED Bioscience/ADAM10/KUZ/MADM/2051/1 Ea
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Anti-ADAM10/KUZ/MADMAntibodyORDERINGINFORMATIONCatalogNo.:2051Format:100uginPBS,pH7.4,0.02%sodiumazide.Purifiedbypeptideimmunoaffinitychromatography.BACKGROUNDTNFα,Notchanditsliganddeltaareallmembrane-boundmoleculeswhicharecleavedbyproteasestoreleasematureproteinsorfunctionalreceptors.ADAM10,ametalloprotease-disintegrininthefamilyofmammalianADAM(foradisintegrinandmetalloprotease)wasrecentlyfoundtocleaveTNFα,Notchanditsliganddelta.Thegenesencodinghuman,mouse,andbovineADAM10havebeenclonedanddesignatedADAM10,Kuzbanian(KUZ),andMADM,respectively.Thesegenesareexpressedinavarietyofhumanandbovinetissues.SPECIFICATIONSUMMARYAntigen:Peptidecorrespondingtoaa732-748ofhumanADAM10.Thissequenceisidenticaltothoseofbovineandratanddiffersfrommousebyoneaminoacid.AccessionNo.:NP_001101HostSpecies:RabbitSpecificity:Human.Inimmunoblotsabandof85kDaisdetectedandmayrepresentprecursor.Lowermwbandsaredetectedinsomecelllines,includingJurkat,andmayrepresenttheprocessedmatureprotein.APPLICATIONSImmunoblotting:useat1-10ug/ml.Immunofluorescence:useat10ug/ml.Immunocytochemistry:useat2ug/ml.WesternblotanalysisofADAM10inJukatwholecelllysate.ImmunofluorescentdetectionofADAM10inK562cells.ImmunochemicaldetectionofADAM10inK562cells.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.DILUTIONINSTRUCTIONSDiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.STORAGEANDSTABILITYThisantibodycanbestoredat4oCforthreemonthsandforoneyearat-20oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
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