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QED Bioscience/Caspase-3/11024-50/1 Ea
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Caspase-3PolyclonalAntibodyORDERINGINFORMATIONCatalognos.:11024-50(50ug)11024-200(200ug)Format:ProteinA-purifiedantibodyinPBS,50%glycerol,0.09%sodiumazide.BACKGROUNDCaspasesarecysteineproteasesthatcleaveaftercertainaspartateresiduesandhavebeenidentifiedasmediatorsofapoptosis.Caspasesaresynthesizedasinactivezymogensthatcanbecleavedtoformactiveenzymesfollowingtheinductionofapoptosisbystressordeathreceptors.Initiatorcaspases(e.g.caspase-8and-10)areactivatedbydimerizationofthezymogenonadedicatedadaptorprotein.Theseactivatedinitiatorcaspasesin-turncleavedownstreameffectororexecutionercaspases(e.g.caspase-3,-6,and-7)inacascade-likemanner,whichcleavekeycellularproteinsthatleadtothemorphologicalchangesassociatedwithapoptoticcelldeath.SPECIFICATIONSUMMARYAntigen:Recombinantfulllengthhumancaspase-3.Accessionno.:P42574GeneID:836HostSpecies:RabbitAntibodyClass:PolyclonalSpecificity:Thisantibodyrecognizescaspase-3inhuman,mouse,rat,bovine,canine,chicken,guineapig,hamster,monkey,pig,rabbit,andyeast.APPLICATIONSImmunoblotting:useat1-5ug/ml.Abandof~36kDaisdetected.Immunohistochemistry:useat10ug/mlDetectionofcaspase-3inhumanspleen.Detectionofcaspase-3inJurkatcelllysate(lane2),staurosporine-treatedJurkatcelllysate(lane3),MCF-7celllysate(negativecontrol,lane4),HeLacelllysate(lane5),ratspleenlysate(lane6),mousespleenlysate(lane7).Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.Caspase-3PolyclonalAntibodyDILUTIONINSTRUCTIONSDiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.STORAGEANDSTABILITYThisproductisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
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