Murine Anti-mCTLA4 mAbAnti-mCTLA4-mIgG2a InvivoFit™Unit sizeCat. codeDocsPrice9D9-derived mouse monoclonal antibody against murine CTLA-49D9-derived mouse monoclonal antibody against murine CTLA-41 mg 10 mg mctla4-mab10-1mctla4-mab10-10TDSMSDSDATATDSMSDSDATA ￥4,586.00Please contact our distributorAdd to favorite ￥29,655.00Please contact our distributorAdd to favoriteAboutSpecificationsContentsDetailsRelated productsRecombinant mouse mAb against murine CTLA-4 for in vivo useEffect of Anti-CTLA4 on T cell activationAnti-mCTLA4-mIgG2a InvivoFit™ is a mouse anti-mouse monoclonal antibody (mAb) featuring the variable region of the previously described anti-mCTLA-4 9D9 mAb [1] and a murine IgG2a constant region. The 9D9 mAb has a sequence that is 100% murine (constant and variable regions), ensuring minimal immunogenicity upon repeated injections into mice. This mAb targets murine cytotoxic T-lymphocyte-associated protein 4 (mCTLA-4) and blocks CTLA4-mediated negative signals downregulating T cell activation.  Thus, anti-mCTLA-4 mAb unleashes T cell activity [1, 2].Notably, the 9D9 mAb, along with most commercially available anti-mCTLA4 mAbs feature the IgG2b isotype which displays low antitumor activity in vivo [2]. To overcome this issue, Anti-mCTLA4-mIgG2a InvivoFit™ was generated by recombinant technology and features the murine IgG2a isotype, which mediates potent cytotoxic functions. Anti-mCTLA4-mIgG2a is provided in an InvivoFit™ grade, a high-quality standard specifically adapted to in vivo studies. Key features of Anti-mCTLA4-mIgG2a InvivoFit™:Sequence is 100% murineFeatures the IgG2a isotype (associated with high antitumor activity)Guaranteed sterile, endotoxin level < 1 EU/mgSpecifically designed for in vivo studies in miceLow aggregation < 5%Produced in both animal-free facilities and defined mediaAnti-mCTLA4-mIgG2a InvivoFit™ is produced in Chinese hamster ovary (CHO) cells, purified by affinity chromatography with protein A, and it"s binding to mCTLA-4 is validated using ELISA. References:1. Selby MJ. et al., 2013. Anti-CTLA-4 antibodies of IgG2a isotype enhance antitumor activity through reduction of intratumoral regulatory T cells. Cancer Immunol Res. 1(1):32-42.2. Ribas A. & Wolchock J.D., 2018. Cancer immunotherapy using checkpoint blockade. Science. 359:1350-55.Figures ELISA binding of Anti-mCTLA4-mIgG2a InvivoFit™ to recombinant murine CTLA-4. A dilution series of Anti-mCTLA4-mIgG2a InvivoFit™ and anti-mCTLA4 (9D9 clone) was used for the detection of coated mCTLA-4 antigen at 5  µg/ml. Detection of bound antibody was performed using an HRP‑conjugated anti‑murine κ-light chain secondary antibody.  HRP activity was assessed by a colorimetric assay using Tetramethylbenzidine (TMB) substrate. Optical density was measured at 490 nm. Data are shown as percentage absorbance. Back to the topSpecificationsSpecificity: Targets cells expressing murine CTLA-4Formulation: Lyophilized from 0.2 μm filtered solution in 150 mM sodium chloride, 20 mM sodium phosphate buffer with 5% saccharoseClonality: Monoclonal antibodyIsotype: Murine IgG2a, kappaSource: CHO cellsPurity: Purified by affinity chromatography with protein ATested application: ELISAQuality control:Binding confirmed using ELISAThe complete sequence of this antibody has been verified< 5% aggregates (confirmed by size exclusion chromatography)Guaranteed sterile and <1 EU/mg (determined by the LAL assay)Back to the topContentsAnti-mCTLA4-mIgG2a InvivoFit™ is provided sterile, endotoxin-free, azide-free and lyophilized.  This product is available in two pack sizes:mctla4-mab10-1​: 1 mgmctla4-mab10-10​: 10 mg  Product is shipped at room temperature. Store lyophilized antibody at -20 °C. Lyophilized product is stable for at least 1 year Avoid repeated freeze-thaw cycles.Back to the topDetailsThe cytotoxic T-lymphocyte-associated protein 4 (CTLA-4, also known as CD152) is an inhibitory receptor expressed on activated T lymphocytes. T-cell activation requires two signals. Signal 1 is delivered upon interaction of the T cell receptor (TCR) with antigenic peptides bound to major histocompatibility complex (MHC) molecules on antigen-presenting cells (APCs). Signal 2 is delivered upon interaction of the co-stimulatory receptor CD28 with the B7 family ligands, B7-1 (CD80) and B7-2 (CD86), on APCs.CTLA-4 is an immune checkpoint molecule upregulated upon T cell activation. It exerts competitive binding to CD28 ligands, thereby preventing T-cell overstimulation. CTLA-4 blockade with monoclonal antibodies (mAbs) has been used to treat patients with metastatic melanoma.Back to the topYou may also needRaji-hCTLA4 CellsHuman lymphoblast cells - ADCC CTLA-4 Target CellsAnti-hCTLA4 isotype familyFamily of antibodies against human CTLA-4Mouse IgG2a Controlβ-galactosidase antibody (isotype control) - Mouse IgG2a