Anti-FLA-STAnti-FLA-STUnit sizeCat. codeDocsPriceDetection and Neutralizing antibody to flagellin from S. typhimuriumCustom size mabg-flastTDSMSDS ￥4,439.00Please contact our distributorAdd to favoriteAboutSpecificationsContentsDetailsCitationsRelated productsJanuary 20th, 2020.This antibody has been removed from our catalog. However, you may contact us if you wish to order minimal quantities.Detection and neutralization antibody to flagellin from S. typhimuriumAnti-FLA-ST is a mouse monoclonal antibody specific against flagellin from Salmonella typhimurium.The antibody has been screened for the ability to neutralize FLA-ST-induced TLR5 activity. Anti-FLA-ST can be used for detection by ELISA and neutralization of purified or recombinant S. typhimurium flagellin.The utility of this antibody for other applications has not been determined.Back to the topSpecificationsClonality: Monoclonal antibodyClone: 18E11Isotype: Mouse IgG1, kappaFormulation: 0.2 µm filtered solution in 68 mM phosphate buffer with 91 mM glycine, 5% w/v saccharose and stabilizing agentsApplication: Neutralization of FLA-ST-induced TLR5 activity, detection by ELISASpecificity: flagellin from Salmonella typhimuriumBack to the topContents100 µg purified anti-FLA-ST provided azide-free and lyophilized Product is shipped at room temperature Store lyophilized antibody at -20°C. Product is stable for 6 months.Back to the topDetailsNeutralization activity of hTLR5-Fc: Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene. After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.Back to the topCitations2019 Nat Commun. DOI: 10.1038/s41467-019-13538-yFlagellin-elicited adaptive immunity suppresses flagellated microbiota and vaccinates against chronic inflammatory diseases.Tran H.Q. et al.You may also needFLA-ST (Standard or Ultrapure)TLR5 Agonist - Flagellin from S. typhimuriumHEK-Blue™ hTLR5Human TLR5 / NF-κB / SEAP reporter HEK293 cellsHEK-Blue™ mTLR5Murine TLR5 / NF-κB / SEAP reporter HEK293 cells