产品说明
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Regulatory status:RUO
Type:Sandwich ELISA, HRP-labelled antibody
Other names:Phagocytic glycoprotein I, Extracellular matrix receptor-III, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, CD44 antigen
Species:Human
Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Urine, Amniotic fluid, Plasma, Cell culture supernatant
Sample Requirements
20 µl (1:60 prediluted)
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.12–4 ng/ml
Limit of Detection
0.015 ng/ml
Intra-assay (Within-Run)
CV = 4.8%
Inter-assay (Run-to-Run)
CV = 4.1%
Spiking Recovery
89,00%
Dilutation Linearity
94,00%
miREIA是一种基于免疫测定的新型miRNA定量方法,涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。
该测定可在常规免疫测定设备上运行,与标准临床工作流程兼容,不需要扩增步骤,并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。
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